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RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage.

Publication ,  Journal Article
Noel, LS; Champion, BR; Holley, CL; Simmons, CJ; Morris, DC; Payne, JA; Lean, JM; Chambers, TJ; Zaman, G; Lanyon, LE; Suva, LJ; Miller, LR
Published in: J Biol Chem
February 13, 1998

Using differential display polymerase chain reaction, we cloned a novel cDNA named RoBo-1 from rat tibia. RoBo-1 is abundantly expressed in bone, including the hypertrophic chondrocytes of the growth plate where cartilage is remodeled into bone. RoBo-1 mRNA expression increased in response to two modulators of bone metabolism, estradiol and intermittent mechanical loading, suggesting a role in bone homeostasis. The 1.6-kilobase cDNA encodes a 240-amino acid protein with a cysteine spacing pattern, suggesting that RoBo-1 is a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family. Furthermore, the C-terminal contains a glycosyl-phosphatidylinositol attachment site, suggesting that it is a cell surface protein similar to other mammalian members of this family. The strongest homology of RoBo-1 is to the snake serum-derived phospholipase A2 inhibitors, which uniquely contain two of the cysteine domains but are secreted proteins. Interestingly, RoBo-1 is likely the first membrane-anchored member of this family containing two cysteine domains. Thus, the tissue specificity, responsiveness to bone protective mediators, along with its relationship to the multifunctional urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family suggests that RoBo-1 may play a novel role in the growth or remodeling of bone.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 13, 1998

Volume

273

Issue

7

Start / End Page

3878 / 3883

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Biosynthesis
  • Molecular Sequence Data
  • In Situ Hybridization
 

Citation

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Noel, L. S., Champion, B. R., Holley, C. L., Simmons, C. J., Morris, D. C., Payne, J. A., … Miller, L. R. (1998). RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage. J Biol Chem, 273(7), 3878–3883. https://doi.org/10.1074/jbc.273.7.3878
Noel, L. S., B. R. Champion, C. L. Holley, C. J. Simmons, D. C. Morris, J. A. Payne, J. M. Lean, et al. “RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage.J Biol Chem 273, no. 7 (February 13, 1998): 3878–83. https://doi.org/10.1074/jbc.273.7.3878.
Noel LS, Champion BR, Holley CL, Simmons CJ, Morris DC, Payne JA, et al. RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage. J Biol Chem. 1998 Feb 13;273(7):3878–83.
Noel, L. S., et al. “RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage.J Biol Chem, vol. 273, no. 7, Feb. 1998, pp. 3878–83. Pubmed, doi:10.1074/jbc.273.7.3878.
Noel LS, Champion BR, Holley CL, Simmons CJ, Morris DC, Payne JA, Lean JM, Chambers TJ, Zaman G, Lanyon LE, Suva LJ, Miller LR. RoBo-1, a novel member of the urokinase plasminogen activator receptor/CD59/Ly-6/snake toxin family selectively expressed in rat bone and growth plate cartilage. J Biol Chem. 1998 Feb 13;273(7):3878–3883.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 13, 1998

Volume

273

Issue

7

Start / End Page

3878 / 3883

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • Receptors, Urokinase Plasminogen Activator
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Biosynthesis
  • Molecular Sequence Data
  • In Situ Hybridization