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Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production.

Publication ,  Journal Article
Bonasia, DE; Martin, JA; Marmotti, A; Amendola, RL; Buckwalter, JA; Rossi, R; Blonna, D; Adkisson, HD; Amendola, A
Published in: Am J Sports Med
November 2011

BACKGROUND: The use of allogenic juvenile chondrocytes or autologous chondral fragments has shown promising laboratory results for the repair of chondral lesions. HYPOTHESIS: Juvenile chondrocytes would not affect matrix production when mixed with adult chondrocytes or cartilage fragments. STUDY DESIGN: Controlled laboratory study. METHODS: Cartilage sources consisted of 3 adult and 3 juvenile (human) donors. In part 1, per each donor, juvenile chondrocytes were mixed with adult chondrocytes in 5 different proportions: 100%, 50%, 25%, 12.5%, and 0%. Three-dimensional cultures in low-melt agarose were performed. At 6 weeks, biochemical and histologic analyses were performed. In part 2, isolated adult, isolated juvenile, and mixed 3-dimensional cultures (1:1) were performed with chondral fragments (<1 mm), both with low-melt agarose and a hyaluronic acid scaffold. At 2 and 6 weeks, cultures were evaluated with biochemical and histologic analyses. RESULTS: Part 1: Biochemical and histologic analyses showed that isolated juvenile cultures performed significantly better than mixed and isolated adult cultures. No significant differences were noted between mixed cultures (1:1) and isolated adult cultures. Part 2: Biochemical and histologic results at 6 weeks showed that mixed cartilage fragment cultures performed better than isolated adult cultures in terms of proteoglycans/DNA ratio (P = .014), percentage of safranin O-positive cells (P = .012), Bern score (P = .001), and collagen type II. No statistically significant difference was noted between juvenile and mixed cultures. CONCLUSION: Extracellular matrix production of juvenile chondrocytes is inhibited by adult chondrocytes. The addition of juvenile cartilage fragments to adult fragments improves matrix production, with a positive interaction between the 2 sources. CLINICAL RELEVANCE: Even if the underlying mechanisms are still unknown, this study describes the behavior of juvenile/adult cocultures using both chondrocytes and cartilage fragments, with potential for new research and clinical applications.

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Published In

Am J Sports Med

DOI

EISSN

1552-3365

Publication Date

November 2011

Volume

39

Issue

11

Start / End Page

2355 / 2361

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Sepharose
  • Proteoglycans
  • Orthopedics
  • Middle Aged
  • Male
  • Hyaluronic Acid
  • Humans
  • Female
  • Extracellular Matrix
 

Citation

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Bonasia, D. E., Martin, J. A., Marmotti, A., Amendola, R. L., Buckwalter, J. A., Rossi, R., … Amendola, A. (2011). Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production. Am J Sports Med, 39(11), 2355–2361. https://doi.org/10.1177/0363546511417172
Bonasia, Davide Edoardo, James A. Martin, Antongiulio Marmotti, Richard L. Amendola, Joseph A. Buckwalter, Roberto Rossi, Davide Blonna, Huston Davis Adkisson, and Annunziato Amendola. “Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production.Am J Sports Med 39, no. 11 (November 2011): 2355–61. https://doi.org/10.1177/0363546511417172.
Bonasia DE, Martin JA, Marmotti A, Amendola RL, Buckwalter JA, Rossi R, et al. Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production. Am J Sports Med. 2011 Nov;39(11):2355–61.
Bonasia, Davide Edoardo, et al. “Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production.Am J Sports Med, vol. 39, no. 11, Nov. 2011, pp. 2355–61. Pubmed, doi:10.1177/0363546511417172.
Bonasia DE, Martin JA, Marmotti A, Amendola RL, Buckwalter JA, Rossi R, Blonna D, Adkisson HD, Amendola A. Cocultures of adult and juvenile chondrocytes compared with adult and juvenile chondral fragments: in vitro matrix production. Am J Sports Med. 2011 Nov;39(11):2355–2361.
Journal cover image

Published In

Am J Sports Med

DOI

EISSN

1552-3365

Publication Date

November 2011

Volume

39

Issue

11

Start / End Page

2355 / 2361

Location

United States

Related Subject Headings

  • Tissue Scaffolds
  • Sepharose
  • Proteoglycans
  • Orthopedics
  • Middle Aged
  • Male
  • Hyaluronic Acid
  • Humans
  • Female
  • Extracellular Matrix