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Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius.

Publication ,  Journal Article
Faherty, SL; Villanueva-Cañas, JL; Klopfer, PH; Albà, MM; Yoder, AD
Published in: Genome biology and evolution
August 2016

Hibernation is a complex physiological response that some mammalian species employ to evade energetic demands. Previous work in mammalian hibernators suggests that hibernation is activated not by a set of genes unique to hibernators, but by differential expression of genes that are present in all mammals. This question of universal genetic mechanisms requires further investigation and can only be tested through additional investigations of phylogenetically dispersed species. To explore this question, we use RNA-Seq to investigate gene expression dynamics as they relate to the varying physiological states experienced throughout the year in a group of primate hibernators-Madagascar's dwarf lemurs (genus Cheirogaleus). In a novel experimental approach, we use longitudinal sampling of biological tissues as a method for capturing gene expression profiles from the same individuals throughout their annual hibernation cycle. We identify 90 candidate genes that have variable expression patterns when comparing two active states (Active 1 and Active 2) with a torpor state. These include genes that are involved in metabolic pathways, feeding behavior, and circadian rhythms, as might be expected to correlate with seasonal physiological state changes. The identified genes appear to be critical for maintaining the health of an animal that undergoes prolonged periods of metabolic depression concurrent with the hibernation phenotype. By focusing on these differentially expressed genes in dwarf lemurs, we compare gene expression patterns in previously studied mammalian hibernators. Additionally, by employing evolutionary rate analysis, we find that hibernation-related genes do not evolve under positive selection in hibernating species relative to nonhibernators.

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Published In

Genome biology and evolution

DOI

EISSN

1759-6653

ISSN

1759-6653

Publication Date

August 2016

Volume

8

Issue

8

Start / End Page

2413 / 2426

Related Subject Headings

  • Seasons
  • RNA
  • Protein Biosynthesis
  • Phylogeny
  • Microarray Analysis
  • Mammals
  • Madagascar
  • Hibernation
  • Gene Expression Regulation
  • Gene Expression Profiling
 

Citation

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ICMJE
MLA
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Faherty, S. L., Villanueva-Cañas, J. L., Klopfer, P. H., Albà, M. M., & Yoder, A. D. (2016). Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius. Genome Biology and Evolution, 8(8), 2413–2426. https://doi.org/10.1093/gbe/evw163
Faherty, Sheena L., José Luis Villanueva-Cañas, Peter H. Klopfer, M Mar Albà, and Anne D. Yoder. “Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius.Genome Biology and Evolution 8, no. 8 (August 2016): 2413–26. https://doi.org/10.1093/gbe/evw163.
Faherty SL, Villanueva-Cañas JL, Klopfer PH, Albà MM, Yoder AD. Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius. Genome biology and evolution. 2016 Aug;8(8):2413–26.
Faherty, Sheena L., et al. “Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius.Genome Biology and Evolution, vol. 8, no. 8, Aug. 2016, pp. 2413–26. Epmc, doi:10.1093/gbe/evw163.
Faherty SL, Villanueva-Cañas JL, Klopfer PH, Albà MM, Yoder AD. Gene Expression Profiling in the Hibernating Primate, Cheirogaleus Medius. Genome biology and evolution. 2016 Aug;8(8):2413–2426.
Journal cover image

Published In

Genome biology and evolution

DOI

EISSN

1759-6653

ISSN

1759-6653

Publication Date

August 2016

Volume

8

Issue

8

Start / End Page

2413 / 2426

Related Subject Headings

  • Seasons
  • RNA
  • Protein Biosynthesis
  • Phylogeny
  • Microarray Analysis
  • Mammals
  • Madagascar
  • Hibernation
  • Gene Expression Regulation
  • Gene Expression Profiling