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How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae).

Publication ,  Journal Article
Martins, TR; Jiang, P; Rausher, MD
Published in: The New phytologist
October 2017

A long-standing question in evolutionary developmental biology is how new traits evolve. Although most floral pigmentation studies have focused on how pigment intensity and composition diversify, few, if any, have explored how a pattern element can shift position. In the present study, we examine the genetic changes underlying shifts in the position of petal spots in Clarkia. Comparative transcriptome analyses were used to identify potential candidate genes responsible for spot formation. Co-segregation analyses in F2 individuals segregating for different spot positions, quantitative PCR, and pyrosequencing, were used to confirm the role of the candidate gene in determining spot position. Transient expression assays were used to identify the expression domain of different alleles. An R2R3Myb transcription factor (CgMyb1) activated spot formation, and different alleles of CgMyb1 were expressed in different domains, leading to spot formation in different petal locations. Reporter assays revealed that promoters from different alleles determine different locations of expression. The evolutionary shift in spot position is due to one or more cis-regulatory changes in the promoter of CgMyb1, indicating that shifts in pattern element position can be caused by changes in a single gene, and that cis-regulatory rewiring can be used to alter the relative position of an existing character.

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Published In

The New phytologist

DOI

EISSN

1469-8137

ISSN

1469-8137

Publication Date

October 2017

Volume

216

Issue

2

Start / End Page

510 / 518

Related Subject Headings

  • Transcription Factors
  • Species Specificity
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Plant Proteins
  • Plant Biology & Botany
  • Phylogeny
  • Luciferases
  • Gene Expression Regulation, Plant
  • Flowers
 

Citation

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Martins, T. R., Jiang, P., & Rausher, M. D. (2017). How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae). The New Phytologist, 216(2), 510–518. https://doi.org/10.1111/nph.14163
Martins, Talline R., Peng Jiang, and Mark D. Rausher. “How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae).The New Phytologist 216, no. 2 (October 2017): 510–18. https://doi.org/10.1111/nph.14163.
Martins TR, Jiang P, Rausher MD. How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae). The New phytologist. 2017 Oct;216(2):510–8.
Martins, Talline R., et al. “How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae).The New Phytologist, vol. 216, no. 2, Oct. 2017, pp. 510–18. Epmc, doi:10.1111/nph.14163.
Martins TR, Jiang P, Rausher MD. How petals change their spots: cis-regulatory re-wiring in Clarkia (Onagraceae). The New phytologist. 2017 Oct;216(2):510–518.
Journal cover image

Published In

The New phytologist

DOI

EISSN

1469-8137

ISSN

1469-8137

Publication Date

October 2017

Volume

216

Issue

2

Start / End Page

510 / 518

Related Subject Headings

  • Transcription Factors
  • Species Specificity
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Plant Proteins
  • Plant Biology & Botany
  • Phylogeny
  • Luciferases
  • Gene Expression Regulation, Plant
  • Flowers