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Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina.

Publication ,  Journal Article
Su, X; Tan, QSW; Parikh, BH; Tan, A; Mehta, MN; Sia Wey, Y; Tun, SBB; Li, L-J; Han, X-Y; Wong, TY; Hunziker, W; Luu, CD; Owada, Y; Zhang, SS ...
Published in: Invest Ophthalmol Vis Sci
June 1, 2016

PURPOSE: To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-). METHODS: Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot. RESULTS: Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression. CONCLUSIONS: Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.

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Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

June 1, 2016

Volume

57

Issue

7

Start / End Page

3397 / 3408

Location

United States

Related Subject Headings

  • Tomography, Optical Coherence
  • Retinal Vessels
  • Retinal Degeneration
  • Retina
  • Real-Time Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Immunohistochemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Su, X., Tan, Q. S. W., Parikh, B. H., Tan, A., Mehta, M. N., Sia Wey, Y., … Chaurasia, S. S. (2016). Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest Ophthalmol Vis Sci, 57(7), 3397–3408. https://doi.org/10.1167/iovs.15-18542
Su, Xinyi, Queenie S. W. Tan, Bhav H. Parikh, Alison Tan, Milan N. Mehta, Yeo Sia Wey, Sai Bo Bo Tun, et al. “Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina.Invest Ophthalmol Vis Sci 57, no. 7 (June 1, 2016): 3397–3408. https://doi.org/10.1167/iovs.15-18542.
Su X, Tan QSW, Parikh BH, Tan A, Mehta MN, Sia Wey Y, et al. Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest Ophthalmol Vis Sci. 2016 Jun 1;57(7):3397–408.
Su, Xinyi, et al. “Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina.Invest Ophthalmol Vis Sci, vol. 57, no. 7, June 2016, pp. 3397–408. Pubmed, doi:10.1167/iovs.15-18542.
Su X, Tan QSW, Parikh BH, Tan A, Mehta MN, Sia Wey Y, Tun SBB, Li L-J, Han X-Y, Wong TY, Hunziker W, Luu CD, Owada Y, Barathi VA, Zhang SS, Chaurasia SS. Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest Ophthalmol Vis Sci. 2016 Jun 1;57(7):3397–3408.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

June 1, 2016

Volume

57

Issue

7

Start / End Page

3397 / 3408

Location

United States

Related Subject Headings

  • Tomography, Optical Coherence
  • Retinal Vessels
  • Retinal Degeneration
  • Retina
  • Real-Time Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Immunohistochemistry