Skip to main content

Use of SMART-generated cDNA for gene expression studies in multiple human tumors.

Publication ,  Journal Article
Zhumabayeva, B; Diatchenko, L; Chenchik, A; Siebert, PD
Published in: Biotechniques
January 2001

We demonstrate here that SMART PCR-amplified cDNAs arrayed on a nylon membrane are suitable for high-throughput tissue expression profiling when starting biological materials are limited. We show that SMART cDNA accurately reflects gene expression patterns found in total RNA by comparing the expression level of several target genes in SMART PCR-amplified cDNAs and their corresponding total RNAs. We also arrayed cDNAs from 68 matched tumor and normal samples on a nylon membrane to determine whether SMART PCR-amplified cDNA could be used for detecting differentially expressed genes in these tissues. These arrays containing normalized tumor and normal cDNAs were hybridized with probes for glutathione peroxidase and gelsolin. The hybridization results revealed cancer-related and patient-specific gene expression differences between tumor and normal tissues for these genes. These studies show that SMART PCR-amplified cDNAs maintain the complexity of the original mRNA population and are thus suitable for high-throughput studies to compare the relative abundance of target genes and to detect differentially expressed genes in a wide variety of tissues simultaneously.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biotechniques

DOI

ISSN

0736-6205

Publication Date

January 2001

Volume

30

Issue

1

Start / End Page

158 / 163

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tyrosine Transaminase
  • Tumor Cells, Cultured
  • Tissue Distribution
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhumabayeva, B., Diatchenko, L., Chenchik, A., & Siebert, P. D. (2001). Use of SMART-generated cDNA for gene expression studies in multiple human tumors. Biotechniques, 30(1), 158–163. https://doi.org/10.2144/01301pf01
Zhumabayeva, B., L. Diatchenko, A. Chenchik, and P. D. Siebert. “Use of SMART-generated cDNA for gene expression studies in multiple human tumors.Biotechniques 30, no. 1 (January 2001): 158–63. https://doi.org/10.2144/01301pf01.
Zhumabayeva B, Diatchenko L, Chenchik A, Siebert PD. Use of SMART-generated cDNA for gene expression studies in multiple human tumors. Biotechniques. 2001 Jan;30(1):158–63.
Zhumabayeva, B., et al. “Use of SMART-generated cDNA for gene expression studies in multiple human tumors.Biotechniques, vol. 30, no. 1, Jan. 2001, pp. 158–63. Pubmed, doi:10.2144/01301pf01.
Zhumabayeva B, Diatchenko L, Chenchik A, Siebert PD. Use of SMART-generated cDNA for gene expression studies in multiple human tumors. Biotechniques. 2001 Jan;30(1):158–163.

Published In

Biotechniques

DOI

ISSN

0736-6205

Publication Date

January 2001

Volume

30

Issue

1

Start / End Page

158 / 163

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tyrosine Transaminase
  • Tumor Cells, Cultured
  • Tissue Distribution
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Neoplasms