Amplification of cDNA ends based on template-switching effect and step-out PCR.
Publication
, Journal Article
Matz, M; Shagin, D; Bogdanova, E; Britanova, O; Lukyanov, S; Diatchenko, L; Chenchik, A
Published in: Nucleic Acids Res
March 15, 1999
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.
Duke Scholars
Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
March 15, 1999
Volume
27
Issue
6
Start / End Page
1558 / 1560
Location
England
Related Subject Headings
- Polymerase Chain Reaction
- Models, Genetic
- Developmental Biology
- DNA, Complementary
- 41 Environmental sciences
- 34 Chemical sciences
- 31 Biological sciences
- 08 Information and Computing Sciences
- 06 Biological Sciences
- 05 Environmental Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Matz, M., Shagin, D., Bogdanova, E., Britanova, O., Lukyanov, S., Diatchenko, L., & Chenchik, A. (1999). Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res, 27(6), 1558–1560. https://doi.org/10.1093/nar/27.6.1558
Matz, M., D. Shagin, E. Bogdanova, O. Britanova, S. Lukyanov, L. Diatchenko, and A. Chenchik. “Amplification of cDNA ends based on template-switching effect and step-out PCR.” Nucleic Acids Res 27, no. 6 (March 15, 1999): 1558–60. https://doi.org/10.1093/nar/27.6.1558.
Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, et al. Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res. 1999 Mar 15;27(6):1558–60.
Matz, M., et al. “Amplification of cDNA ends based on template-switching effect and step-out PCR.” Nucleic Acids Res, vol. 27, no. 6, Mar. 1999, pp. 1558–60. Pubmed, doi:10.1093/nar/27.6.1558.
Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A. Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res. 1999 Mar 15;27(6):1558–1560.
Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
March 15, 1999
Volume
27
Issue
6
Start / End Page
1558 / 1560
Location
England
Related Subject Headings
- Polymerase Chain Reaction
- Models, Genetic
- Developmental Biology
- DNA, Complementary
- 41 Environmental sciences
- 34 Chemical sciences
- 31 Biological sciences
- 08 Information and Computing Sciences
- 06 Biological Sciences
- 05 Environmental Sciences