Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.
Publication
, Journal Article
Lukyanov, K; Diatchenko, L; Chenchik, A; Nanisetti, A; Siebert, P; Usman, N; Matz, M; Lukyanov, S
Published in: Biochem Biophys Res Commun
January 13, 1997
Here we describe a method for preparing high-quality cDNA libraries from total RNA. By this method, double-stranded (ds) cDNA ligated with a specially designed ds adaptor is amplified by PCR using a modified T-primer and another primer corresponding to the outer part of the adaptor. The suppression PCR effect strongly inhibits the amplification of poly(A) RNA, thereby reducing background. This method leads to amplification of high-quality cDNA, facilitating the construction of representative cDNA libraries from as little as 10-100 ng of total RNA.
Duke Scholars
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
January 13, 1997
Volume
230
Issue
2
Start / End Page
285 / 288
Location
United States
Related Subject Headings
- RNA
- Polymerase Chain Reaction
- Muscle, Skeletal
- Humans
- Gene Library
- Electrophoresis, Agar Gel
- DNA, Complementary
- Biochemistry & Molecular Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Lukyanov, K., Diatchenko, L., Chenchik, A., Nanisetti, A., Siebert, P., Usman, N., … Lukyanov, S. (1997). Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochem Biophys Res Commun, 230(2), 285–288. https://doi.org/10.1006/bbrc.1996.5948
Lukyanov, K., L. Diatchenko, A. Chenchik, A. Nanisetti, P. Siebert, N. Usman, M. Matz, and S. Lukyanov. “Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.” Biochem Biophys Res Commun 230, no. 2 (January 13, 1997): 285–88. https://doi.org/10.1006/bbrc.1996.5948.
Lukyanov K, Diatchenko L, Chenchik A, Nanisetti A, Siebert P, Usman N, et al. Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochem Biophys Res Commun. 1997 Jan 13;230(2):285–8.
Lukyanov, K., et al. “Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect.” Biochem Biophys Res Commun, vol. 230, no. 2, Jan. 1997, pp. 285–88. Pubmed, doi:10.1006/bbrc.1996.5948.
Lukyanov K, Diatchenko L, Chenchik A, Nanisetti A, Siebert P, Usman N, Matz M, Lukyanov S. Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect. Biochem Biophys Res Commun. 1997 Jan 13;230(2):285–288.
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
January 13, 1997
Volume
230
Issue
2
Start / End Page
285 / 288
Location
United States
Related Subject Headings
- RNA
- Polymerase Chain Reaction
- Muscle, Skeletal
- Humans
- Gene Library
- Electrophoresis, Agar Gel
- DNA, Complementary
- Biochemistry & Molecular Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology