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Characterization and tissue expression of multiple triiodothyronine receptor-auxiliary proteins and their relationship to the retinoid X-receptors.

Publication ,  Journal Article
Sugawara, A; Yen, PM; Darling, DS; Chin, WW
Published in: Endocrinology
September 1993

Thyroid hormone receptor (TR) binding to thyroid hormone response elements is enhanced by heterodimerization with T3 receptor auxiliary proteins (TRAPs). Although retinoid X-receptors (RXRs) behave similarly to TRAP by heterodimerizing with TRs and enhancing TR binding to thyroid hormone response elements, it is not known whether endogenous TRAPs are RXRs. In this study, we used the electrophoretic mobility shift assay to demonstrate that at least two different TR-TRAP heterodimer complexes can be formed from nuclear extracts of various rat tissues and cell lines. Additionally, the TRAPs appear to be differentially expressed in rat tissues. In antibody supershift experiments, most of the faster migrating TR-TRAP heterodimer bands and some of the slower migrating TR-TRAP heterodimer bands were recognized in several tissues and cell lines by the anti-RXR alpha-specific antibody. Immunodepletion assays showed that the slower migrating TR-TRAP heterodimer bands in most tissues and cell lines were recognized by the anti-RXR beta-specific antibody. Therefore, we have demonstrated that RXR alpha and RXR beta are endogenous TRAPs in a variety of tissues and cell lines, and are differentially expressed. We speculate that this heterogeneity of TRAP distribution may contribute to tissue and cell-specific expression of T3-regulated genes.

Duke Scholars

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

September 1993

Volume

133

Issue

3

Start / End Page

965 / 971

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Retinoid X Receptors
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Thyroid Hormone
  • Receptors, Retinoic Acid
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • Organ Specificity
 

Citation

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Sugawara, A., Yen, P. M., Darling, D. S., & Chin, W. W. (1993). Characterization and tissue expression of multiple triiodothyronine receptor-auxiliary proteins and their relationship to the retinoid X-receptors. Endocrinology, 133(3), 965–971. https://doi.org/10.1210/endo.133.3.8396023
Sugawara, A., P. M. Yen, D. S. Darling, and W. W. Chin. “Characterization and tissue expression of multiple triiodothyronine receptor-auxiliary proteins and their relationship to the retinoid X-receptors.Endocrinology 133, no. 3 (September 1993): 965–71. https://doi.org/10.1210/endo.133.3.8396023.
Sugawara, A., et al. “Characterization and tissue expression of multiple triiodothyronine receptor-auxiliary proteins and their relationship to the retinoid X-receptors.Endocrinology, vol. 133, no. 3, Sept. 1993, pp. 965–71. Pubmed, doi:10.1210/endo.133.3.8396023.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

September 1993

Volume

133

Issue

3

Start / End Page

965 / 971

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Retinoid X Receptors
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Thyroid Hormone
  • Receptors, Retinoic Acid
  • Receptors, Cell Surface
  • Rats, Sprague-Dawley
  • Rats
  • Organ Specificity