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Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells.

Publication ,  Journal Article
Christie, BR; Franks, KM; Seamans, JK; Saga, K; Sejnowski, TJ
Published in: Hippocampus
2000

Long-term potentiation (LTP) of synaptic efficacy was examined in interneurons and giant cells in the stratum radiatum region of the hippocampal CA1 subfield. Cells were visually selected using differential interference contrast (DIC) optics and filled with biocytin while being recorded using whole-cell patch-clamp techniques. Electrophysiological criteria, including spike height, width, and degree of spike adaptation shown to sustained depolarization, proved inadequate for differentiating interneurons from giant cells. We found that cells in the stratum radiatum, however, could be reliably differentiated using DIC optics or following intracellular staining. The response of the two cell types to tetanic stimulation further dissociated them. Long-term potentiation, dependent on the activation of NMDAr (N-methyl-D-aspartate receptors), could reliably be induced in interneurons with stimuli administered at 200 Hz, but not 100 Hz. Giant cells, in contrast, exhibited NMDA receptor-dependent LTP in response to 100-Hz stimuli, but not the 200-Hz stimuli. LTP induction in interneurons also appeared temperature-dependent, being much more robust at 34 degrees C than at room temperature. The LTP in both cell types required postsynaptic calcium influx, and was not due to the passive propagation of LTP induction in neighboring pyramidal cells. These results suggest that different cell types within the hippocampal formation may preferentially alter synaptic connectivity in a frequency-specific manner.

Duke Scholars

Published In

Hippocampus

DOI

ISSN

1050-9631

Publication Date

2000

Volume

10

Issue

6

Start / End Page

673 / 683

Location

United States

Related Subject Headings

  • Synapses
  • Receptors, N-Methyl-D-Aspartate
  • Rats, Sprague-Dawley
  • Rats
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Neural Pathways
  • Neural Inhibition
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Christie, B. R., Franks, K. M., Seamans, J. K., Saga, K., & Sejnowski, T. J. (2000). Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells. Hippocampus, 10(6), 673–683. https://doi.org/10.1002/1098-1063(2000)10:6<673::AID-HIPO1005>3.0.CO;2-O
Christie, B. R., K. M. Franks, J. K. Seamans, K. Saga, and T. J. Sejnowski. “Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells.Hippocampus 10, no. 6 (2000): 673–83. https://doi.org/10.1002/1098-1063(2000)10:6<673::AID-HIPO1005>3.0.CO;2-O.
Christie BR, Franks KM, Seamans JK, Saga K, Sejnowski TJ. Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells. Hippocampus. 2000;10(6):673–83.
Christie, B. R., et al. “Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells.Hippocampus, vol. 10, no. 6, 2000, pp. 673–83. Pubmed, doi:10.1002/1098-1063(2000)10:6<673::AID-HIPO1005>3.0.CO;2-O.
Christie BR, Franks KM, Seamans JK, Saga K, Sejnowski TJ. Synaptic plasticity in morphologically identified CA1 stratum radiatum interneurons and giant projection cells. Hippocampus. 2000;10(6):673–683.
Journal cover image

Published In

Hippocampus

DOI

ISSN

1050-9631

Publication Date

2000

Volume

10

Issue

6

Start / End Page

673 / 683

Location

United States

Related Subject Headings

  • Synapses
  • Receptors, N-Methyl-D-Aspartate
  • Rats, Sprague-Dawley
  • Rats
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Neuronal Plasticity
  • Neurology & Neurosurgery
  • Neural Pathways
  • Neural Inhibition