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Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes.

Publication ,  Journal Article
Li, J; Chen, Z; Stang, MT; Gao, W
Published in: Autophagy
February 2017

The membrane source for autophagosome biogenesis is an unsolved mystery in the study of autophagy. ATG16L1 forms a complex with ATG12-ATG5 (the ATG16L1 complex). The ATG16L1 complex is recruited to autophagic membranes to convert MAP1LC3B-I to MAP1LC3B-II. The ATG16L1 complex dissociates from the phagophore before autophagosome membrane closure. Thus, ATG16L1 can be used as an early event marker for the study of autophagosome biogenesis. We found that among 3 proteins in the ATG16L1 complex, only ATG16L1 formed puncta-like structures when transiently overexpressed. ATG16L1+ puncta formed by transient expression could represent autophagic membrane structures. We thoroughly characterized the transiently expressed ATG16L1 in several mammalian cell lines. We found that transient expression of ATG16L1 not only inhibited autophagosome biogenesis, but also aberrantly targeted RAB11-positive recycling endosomes, resulting in recycling endosome aggregates. We conclude that transient expression of ATG16L1 is not a physiological model for the study of autophagy. Caution is warranted when reviewing findings derived from a transient expression model of ATG16L1.

Duke Scholars

Published In

Autophagy

DOI

EISSN

1554-8635

Publication Date

February 2017

Volume

13

Issue

2

Start / End Page

345 / 358

Location

United States

Related Subject Headings

  • rab GTP-Binding Proteins
  • Protein Transport
  • Phosphatidylinositol 3-Kinases
  • Microtubule-Associated Proteins
  • Mice
  • Humans
  • Hela Cells
  • HeLa Cells
  • Green Fluorescent Proteins
  • Endosomes
 

Citation

APA
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ICMJE
MLA
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Li, J., Chen, Z., Stang, M. T., & Gao, W. (2017). Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes. Autophagy, 13(2), 345–358. https://doi.org/10.1080/15548627.2016.1256521
Li, Jiehua, Zhixia Chen, Michael T. Stang, and Wentao Gao. “Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes.Autophagy 13, no. 2 (February 2017): 345–58. https://doi.org/10.1080/15548627.2016.1256521.
Li, Jiehua, et al. “Transiently expressed ATG16L1 inhibits autophagosome biogenesis and aberrantly targets RAB11-positive recycling endosomes.Autophagy, vol. 13, no. 2, Feb. 2017, pp. 345–58. Pubmed, doi:10.1080/15548627.2016.1256521.

Published In

Autophagy

DOI

EISSN

1554-8635

Publication Date

February 2017

Volume

13

Issue

2

Start / End Page

345 / 358

Location

United States

Related Subject Headings

  • rab GTP-Binding Proteins
  • Protein Transport
  • Phosphatidylinositol 3-Kinases
  • Microtubule-Associated Proteins
  • Mice
  • Humans
  • Hela Cells
  • HeLa Cells
  • Green Fluorescent Proteins
  • Endosomes