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MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration.

Publication ,  Journal Article
Akagi-Kurashige, Y; Yamashiro, K; Gotoh, N; Miyake, M; Morooka, S; Yoshikawa, M; Nakata, I; Kumagai, K; Tsujikawa, A; Yamada, R; Matsuda, F ...
Published in: Ophthalmology
November 2015

PURPOSE: Age-related macular degeneration (AMD) is the leading cause of severe visual impairment. Despite treatment, a central scotoma often remains. The size of the scotoma depends on the lesion size of the choroidal neovascular membrane and significantly affects the patient's quality of life, and the lesion size of neovascularization also affects response to treatments. The aim of this study was to identify genes associated with the neovascular lesion size in neovascular AMD. DESIGN: A genome-wide association study (GWAS). PARTICIPANTS: We included 1146 Japanese patients with neovascular AMD. METHODS: We performed a 2-stage GWAS for the lesion size of AMD as a quantitative trait among 1146 (first stage: 727, second stage: 419) Japanese patients with neovascular AMD. Lesion size was determined by the greatest linear dimension measured with fluorescein angiography examination before treatment. We examined the association between the genotypic distribution of each single nucleotide polymorphism (SNP) and the trait using an additive model adjusted for age and sex. To evaluate the associations between AMD development and SNPs associated with lesion size, we also performed a case-control study by using the genotype data from these 1146 Japanese patients as case subjects and the fixed dataset from the Nagahama Study as control subjects. MAIN OUTCOME MEASURES: Genes associated with the lesion size in neovascular AMD. RESULTS: In the discovery stage, rs10895322 in MMP20 showed a genome-wide significant P value of 6.95×10(-8), and rs2284665 in ARMS2/HTRA1 showed a P value of 1.55×10(-7). The associations of these 2 SNPs were successfully replicated in the replication stage, and a meta-analysis of both stages showed genome-wide significant P values (2.80×10(-9) and 4.41×10(-9), respectively). In a case-control study using 3248 Japanese subjects as controls, we could not find contribution of MMP20 rs10895322 for AMD development. Although MMP20 has been thought to be expressed only in dental tissues, we confirmed MMP20 expression in the human retina and retinal pigment epithelium/choroid with polymerase chain reaction. CONCLUSIONS: The growth of choroidal neovascularization in AMD would be affected by 2 genes: MMP20, a newly confirmed gene expressed in the retina, and ARMS2/HTRA1, a well-known susceptibility gene for AMD.

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Published In

Ophthalmology

DOI

EISSN

1549-4713

Publication Date

November 2015

Volume

122

Issue

11

Start / End Page

2295 / 2302.e2

Location

United States

Related Subject Headings

  • Wet Macular Degeneration
  • Serine Endopeptidases
  • Scotoma
  • Proteins
  • Polymorphism, Single Nucleotide
  • Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Matrix Metalloproteinase 20
  • Male
  • Japan
 

Citation

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Akagi-Kurashige, Y., Yamashiro, K., Gotoh, N., Miyake, M., Morooka, S., Yoshikawa, M., … Nagahama Cohort Research Group, . (2015). MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration. Ophthalmology, 122(11), 2295-2302.e2. https://doi.org/10.1016/j.ophtha.2015.07.032
Akagi-Kurashige, Yumiko, Kenji Yamashiro, Norimoto Gotoh, Masahiro Miyake, Satoshi Morooka, Munemitsu Yoshikawa, Isao Nakata, et al. “MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration.Ophthalmology 122, no. 11 (November 2015): 2295-2302.e2. https://doi.org/10.1016/j.ophtha.2015.07.032.
Akagi-Kurashige Y, Yamashiro K, Gotoh N, Miyake M, Morooka S, Yoshikawa M, et al. MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration. Ophthalmology. 2015 Nov;122(11):2295-2302.e2.
Akagi-Kurashige, Yumiko, et al. “MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration.Ophthalmology, vol. 122, no. 11, Nov. 2015, pp. 2295-2302.e2. Pubmed, doi:10.1016/j.ophtha.2015.07.032.
Akagi-Kurashige Y, Yamashiro K, Gotoh N, Miyake M, Morooka S, Yoshikawa M, Nakata I, Kumagai K, Tsujikawa A, Yamada R, Matsuda F, Saito M, Iida T, Sugahara M, Kurimoto Y, Cheng C-Y, Khor C-C, Wong T-Y, Yoshimura N, Nagahama Cohort Research Group. MMP20 and ARMS2/HTRA1 Are Associated with Neovascular Lesion Size in Age-Related Macular Degeneration. Ophthalmology. 2015 Nov;122(11):2295-2302.e2.
Journal cover image

Published In

Ophthalmology

DOI

EISSN

1549-4713

Publication Date

November 2015

Volume

122

Issue

11

Start / End Page

2295 / 2302.e2

Location

United States

Related Subject Headings

  • Wet Macular Degeneration
  • Serine Endopeptidases
  • Scotoma
  • Proteins
  • Polymorphism, Single Nucleotide
  • Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Matrix Metalloproteinase 20
  • Male
  • Japan