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N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein.

Publication ,  Journal Article
Liu, N; Zhou, KI; Parisien, M; Dai, Q; Diatchenko, L; Pan, T
Published in: Nucleic Acids Res
June 2, 2017

N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic messenger RNA (mRNA), and affects almost every stage of the mRNA life cycle. The YTH-domain proteins can specifically recognize m6A modification to control mRNA maturation, translation and decay. m6A can also alter RNA structures to affect RNA-protein interactions in cells. Here, we show that m6A increases the accessibility of its surrounding RNA sequence to bind heterogeneous nuclear ribonucleoprotein G (HNRNPG). Furthermore, HNRNPG binds m6A-methylated RNAs through its C-terminal low-complexity region, which self-assembles into large particles in vitro. The Arg-Gly-Gly repeats within the low-complexity region are required for binding to the RNA motif exposed by m6A methylation. We identified 13,191 m6A sites in the transcriptome that regulate RNA-HNRNPG interaction and thereby alter the expression and alternative splicing pattern of target mRNAs. Low-complexity regions are pervasive among mRNA binding proteins. Our results show that m6A-dependent RNA structural alterations can promote direct binding of m6A-modified RNAs to low-complexity regions in RNA binding proteins.

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Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

June 2, 2017

Volume

45

Issue

10

Start / End Page

6051 / 6063

Location

England

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • RNA, Small Interfering
  • RNA, Long Noncoding
  • RNA Interference
  • RNA
  • Protein Binding
  • Phylogeny
  • Oligoribonucleotides
  • Nucleic Acid Conformation
 

Citation

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Liu, N., Zhou, K. I., Parisien, M., Dai, Q., Diatchenko, L., & Pan, T. (2017). N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Nucleic Acids Res, 45(10), 6051–6063. https://doi.org/10.1093/nar/gkx141
Liu, Nian, Katherine I. Zhou, Marc Parisien, Qing Dai, Luda Diatchenko, and Tao Pan. “N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein.Nucleic Acids Res 45, no. 10 (June 2, 2017): 6051–63. https://doi.org/10.1093/nar/gkx141.
Liu N, Zhou KI, Parisien M, Dai Q, Diatchenko L, Pan T. N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Nucleic Acids Res. 2017 Jun 2;45(10):6051–63.
Liu, Nian, et al. “N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein.Nucleic Acids Res, vol. 45, no. 10, June 2017, pp. 6051–63. Pubmed, doi:10.1093/nar/gkx141.
Liu N, Zhou KI, Parisien M, Dai Q, Diatchenko L, Pan T. N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Nucleic Acids Res. 2017 Jun 2;45(10):6051–6063.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

June 2, 2017

Volume

45

Issue

10

Start / End Page

6051 / 6063

Location

England

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • RNA, Small Interfering
  • RNA, Long Noncoding
  • RNA Interference
  • RNA
  • Protein Binding
  • Phylogeny
  • Oligoribonucleotides
  • Nucleic Acid Conformation