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SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples

Publication ,  Conference
Crawford, BM; Wang, HN; Fales, AM; Bowie, ML; Seewaldt, VL; Vo-Dinh, T
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
January 1, 2017

The development of sensitive and selective biosensing techniques is of great interest for clinical diagnostics. Here, we describe the development and application of a surface enhanced Raman scattering (SERS) sensing technology, referred to as "inverse Molecular Sentinel (iMS)" nanoprobes, for the detection of nucleic acid biomarkers in biological samples. This iMS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform for a homogenous assay for multiplexed detection of nucleic acid biomarkers, including DNA, RNA and microRNA (miRNA). The "OFF-to-ON" signal switch is based on a non-enzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. Here, we demonstrate the development of iMS nanoprobes for the detection of DNA sequences as well as a modified design of the nanoprobe for the detection of short (22-nt) microRNA sequences. The application of iMS nanoprobes to detect miRNAs in real biological samples was performed with total small RNA extracted from breast cancer cell lines. The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer. The results of this study demonstrate the feasibility of applying the iMS technique for multiplexed detection of nucleic acid biomarkers, including short miRNAs molecules.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

January 1, 2017

Volume

10080
 

Citation

APA
Chicago
ICMJE
MLA
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Crawford, B. M., Wang, H. N., Fales, A. M., Bowie, M. L., Seewaldt, V. L., & Vo-Dinh, T. (2017). SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 10080). https://doi.org/10.1117/12.2258272
Crawford, B. M., H. N. Wang, A. M. Fales, M. L. Bowie, V. L. Seewaldt, and T. Vo-Dinh. “SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 10080, 2017. https://doi.org/10.1117/12.2258272.
Crawford BM, Wang HN, Fales AM, Bowie ML, Seewaldt VL, Vo-Dinh T. SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2017.
Crawford, B. M., et al. “SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10080, 2017. Scopus, doi:10.1117/12.2258272.
Crawford BM, Wang HN, Fales AM, Bowie ML, Seewaldt VL, Vo-Dinh T. SERS-based inverse molecular sentinel (iMS) nanoprobes for multiplexed detection of microRNA cancer biomarkers in biological samples. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2017.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

January 1, 2017

Volume

10080