Uptake and Distribution of Silver in the Aquatic Plant Landoltia punctata (Duckweed) Exposed to Silver and Silver Sulfide Nanoparticles.

Aquatic ecosystems are expected to receive Ag⁰ and Ag₂S nanoparticles (NPs) through anthropogenic waste streams. The speciation of silver in Ag-NPs affects their fate in ecosystems, but its influence on interactions with aquatic plants is still unclear. Here, the Ag speciation and distribution was measured in an aquatic plant, duckweed (Landoltia punctata), exposed to Ag⁰ or Ag₂S NPs, or to AgNO₃. The silver distribution in duckweed roots was visualized using synchrotron-based micro X-ray fluorescence (XRF) mapping and Ag speciation was determined using extended X-ray absorption fine structure (EXAFS) spectroscopy. Duckweed exposed to Ag₂S-NPs or Ag⁰-NPs accumulated similar Ag concentrations despite an order of magnitude smaller dissolved Ag fraction measured in the exposure medium for Ag₂S-NPs compared to Ag⁰-NPs. By 24 h after exposure, all three forms of silver had accumulated on and partially in the roots regardless of the form of Ag exposed to the plants. Once associated with duckweed tissue, Ag⁰-NPs had transformed primarily into silver sulfide and silver thiol species. This suggests that plant defenses were active within or at the root surface. The Ag₂S-NPs remained as Ag₂S, while AgNO₃ exposure led to Ag⁰ and sulfur-associated Ag species in plant tissue. Thus, regardless of initial speciation, Ag was readily available to duckweed.

Duke Scholars

Author Mark Wiesner Civil and Environmental Engineering