Skip to main content
release_alert
Welcome to the new Scholars 3.0! Read about new features and let us know what you think.
cancel

Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity.

Publication ,  Journal Article
Tsai, Y-S; Tsai, P-J; Jiang, M-J; Chou, T-Y; Pendse, A; Kim, H-S; Maeda, N
Published in: Mol Endocrinol
November 2009

Mutations and polymorphisms in PPARG have been linked to adiposity and partial lipodystrophy in humans. However, how disturbances in PPARG lead to depot-specific effects on adipose tissue, as shown by the characteristic aberrant fat distribution in patients, remains unclear. By manipulating the 3'-untranslated region of the Pparg gene, we have generated mice with peroxisome proliferator-activated receptor gamma (PPAR gamma) gene expression ranging from 25% to 100% normal. Basal levels of PPAR gamma transcripts between 50% and approximately 100% had no significant effect on body weight, fat mass, and insulin sensitivity. In contrast, mice with 25% normal PPAR gamma expression exhibited reduced body weight and total fat mass, insulin resistance, and dyslipidemia. Interestingly, fat mass was selectively reduced in perigonadal depot without significant changes in inguinal and other depots. Expression of adipogenic factor CCAAT enhancer binding protein-alpha and some other metabolic genes containing peroxisome proliferator response element were reduced in a perigonadal depot-specific fashion. This was further associated with depot-specific reduction in the expression of adipokines, increased expression of TNFalpha, and increased ectopic lipid deposition in muscles. Together, these results underscore the differential sensitivity of the individual fat depots on PPAR gamma availability as an underlying mechanism of partial lipodystrophy.

Duke Scholars

Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

November 2009

Volume

23

Issue

11

Start / End Page

1787 / 1798

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • PPAR gamma
  • Mice, Transgenic
  • Mice
  • Male
  • Lipodystrophy
  • Lipid Metabolism
  • Insulin Resistance
  • Gonads
  • Glucose Tolerance Test
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tsai, Y.-S., Tsai, P.-J., Jiang, M.-J., Chou, T.-Y., Pendse, A., Kim, H.-S., & Maeda, N. (2009). Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity. Mol Endocrinol, 23(11), 1787–1798. https://doi.org/10.1210/me.2009-0073
Tsai, Yau-Sheng, Pei-Jane Tsai, Man-Jin Jiang, Ting-Yu Chou, Avani Pendse, Hyung-Suk Kim, and Nobuyo Maeda. “Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity.Mol Endocrinol 23, no. 11 (November 2009): 1787–98. https://doi.org/10.1210/me.2009-0073.
Tsai Y-S, Tsai P-J, Jiang M-J, Chou T-Y, Pendse A, Kim H-S, et al. Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity. Mol Endocrinol. 2009 Nov;23(11):1787–98.
Tsai, Yau-Sheng, et al. “Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity.Mol Endocrinol, vol. 23, no. 11, Nov. 2009, pp. 1787–98. Pubmed, doi:10.1210/me.2009-0073.
Tsai Y-S, Tsai P-J, Jiang M-J, Chou T-Y, Pendse A, Kim H-S, Maeda N. Decreased PPAR gamma expression compromises perigonadal-specific fat deposition and insulin sensitivity. Mol Endocrinol. 2009 Nov;23(11):1787–1798.

Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

November 2009

Volume

23

Issue

11

Start / End Page

1787 / 1798

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • PPAR gamma
  • Mice, Transgenic
  • Mice
  • Male
  • Lipodystrophy
  • Lipid Metabolism
  • Insulin Resistance
  • Gonads
  • Glucose Tolerance Test