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High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast.

Publication ,  Journal Article
Yin, Y; Dominska, M; Yim, E; Petes, TD
Published in: Elife
July 17, 2017

In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

July 17, 2017

Volume

6

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Nucleic Acid Heteroduplexes
  • Neoplastic Syndromes, Hereditary
  • MutL Protein Homolog 1
  • Mitosis
  • DNA, Fungal
  • Colorectal Neoplasms
 

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Yin, Y., Dominska, M., Yim, E., & Petes, T. D. (2017). High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast. Elife, 6. https://doi.org/10.7554/eLife.28069
Yin, Yi, Margaret Dominska, Eunice Yim, and Thomas D. Petes. “High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast.Elife 6 (July 17, 2017). https://doi.org/10.7554/eLife.28069.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

July 17, 2017

Volume

6

Location

England

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Nucleic Acid Heteroduplexes
  • Neoplastic Syndromes, Hereditary
  • MutL Protein Homolog 1
  • Mitosis
  • DNA, Fungal
  • Colorectal Neoplasms