Scholars@Duke publication: The effect of influenza B virus M segment on the replication of reassortant vaccine viruses

The effect of influenza B virus M segment on the replication of reassortant vaccine viruses

Publication , Journal Article

Chen, Z; Du, J; Hoffmann, E; Spaete, J; Kemble, G

Published in: International Congress Series

June 1, 2004

Flumist™ is a cold-adapted (ca), live attenuated influenza virus vaccine containing H1N1, H3N2, and B strains. The vaccine strains have a 6:2 gene constellation. Six gene segments, PB1, PB2, PA, NP, M and NS, are derived from the master donor viruses, ca A/Ann Arbor/6/60(MDV-A) and ca B/Ann Arbor/1/66(MDV-B), and two gene segments, the hemagglutinin (HA) and neuraminidase (NA), are derived from circulating wild-type strains. We observed that the M gene segment of influenza B virus reassortants can influence the replication of these viruses in CEK cells. Replication curves revealed that a 6:2 reassortant B virus (wt HA and wt NA) replicated less well, by approximately 2 log10, than the corresponding 5:3 strain (wt HA, wt NA and wt M) at 33 °C in chicken embryo kidney (CEK) cells; this difference was not observed at 25 °C. © 2004, Elsevier B.V.

Duke Scholars

Author Joshua Paul Spaete Medicine, Gastroenterology

Published In

International Congress Series

DOI

10.1016/j.ics.2004.02.048

ISSN

0531-5131

Publication Date

June 1, 2004

Volume

1263

Issue

Start / End Page

435 / 438

Citation

APA

Chicago

ICMJE

MLA

NLM

Chen, Z., Du, J., Hoffmann, E., Spaete, J., & Kemble, G. (2004). The effect of influenza B virus M segment on the replication of reassortant vaccine viruses. International Congress Series, 1263(C), 435–438. https://doi.org/10.1016/j.ics.2004.02.048

Chen, Z., J. Du, E. Hoffmann, J. Spaete, and G. Kemble. “The effect of influenza B virus M segment on the replication of reassortant vaccine viruses.” International Congress Series 1263, no. C (June 1, 2004): 435–38. https://doi.org/10.1016/j.ics.2004.02.048.

Chen Z, Du J, Hoffmann E, Spaete J, Kemble G. The effect of influenza B virus M segment on the replication of reassortant vaccine viruses. International Congress Series. 2004 Jun 1;1263(C):435–8.

Chen, Z., et al. “The effect of influenza B virus M segment on the replication of reassortant vaccine viruses.” International Congress Series, vol. 1263, no. C, June 2004, pp. 435–38. Scopus, doi:10.1016/j.ics.2004.02.048.

Chen Z, Du J, Hoffmann E, Spaete J, Kemble G. The effect of influenza B virus M segment on the replication of reassortant vaccine viruses. International Congress Series. 2004 Jun 1;1263(C):435–438.

Published In

International Congress Series

DOI

10.1016/j.ics.2004.02.048

ISSN

0531-5131

Publication Date

June 1, 2004

Volume

1263

Issue

Start / End Page

435 / 438