Skip to main content
Journal cover image

Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

Publication ,  Journal Article
Goodwin, CR; Woodard, CL; Zhou, X; Pan, J; Olivi, A; Xia, S; Bettegowda, C; Sciubba, DM; Pevsner, J; Zhu, H; Laterra, J
Published in: Transl Oncol
April 2016

Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Transl Oncol

DOI

ISSN

1936-5233

Publication Date

April 2016

Volume

9

Issue

2

Start / End Page

124 / 129

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Goodwin, C. R., Woodard, C. L., Zhou, X., Pan, J., Olivi, A., Xia, S., … Laterra, J. (2016). Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems. Transl Oncol, 9(2), 124–129. https://doi.org/10.1016/j.tranon.2016.02.001
Goodwin, C Rory, Crystal L. Woodard, Xin Zhou, Jianbo Pan, Alessandro Olivi, Shuli Xia, Chetan Bettegowda, et al. “Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.Transl Oncol 9, no. 2 (April 2016): 124–29. https://doi.org/10.1016/j.tranon.2016.02.001.
Goodwin CR, Woodard CL, Zhou X, Pan J, Olivi A, Xia S, et al. Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems. Transl Oncol. 2016 Apr;9(2):124–9.
Goodwin, C. Rory, et al. “Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.Transl Oncol, vol. 9, no. 2, Apr. 2016, pp. 124–29. Pubmed, doi:10.1016/j.tranon.2016.02.001.
Goodwin CR, Woodard CL, Zhou X, Pan J, Olivi A, Xia S, Bettegowda C, Sciubba DM, Pevsner J, Zhu H, Laterra J. Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems. Transl Oncol. 2016 Apr;9(2):124–129.
Journal cover image

Published In

Transl Oncol

DOI

ISSN

1936-5233

Publication Date

April 2016

Volume

9

Issue

2

Start / End Page

124 / 129

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis
  • 1103 Clinical Sciences
  • 0601 Biochemistry and Cell Biology