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Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.

Publication ,  Journal Article
Wong, K; Ren, XR; Huang, YZ; Xie, Y; Liu, G; Saito, H; Tang, H; Wen, L; Brady-Kalnay, SM; Mei, L; Wu, JY; Xiong, WC; Rao, Y
Published in: Cell
October 19, 2001

The Slit protein guides neuronal and leukocyte migration through the transmembrane receptor Roundabout (Robo). We report here that the intracellular domain of Robo interacts with a novel family of Rho GTPase activating proteins (GAPs). Two of the Slit-Robo GAPs (srGAPs) are expressed in regions responsive to Slit. Slit increased srGAP1-Robo1 interaction and inactivated Cdc42. A dominant negative srGAP1 blocked Slit inactivation of Cdc42 and Slit repulsion of migratory cells from the anterior subventricular zone (SVZa) of the forebrain. A constitutively active Cdc42 blocked the repulsive effect of Slit. These results have demonstrated important roles for GAPs and Cdc42 in neuronal migration. We propose a signal transduction pathway from the extracellular guidance cue to intracellular actin polymerization.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 19, 2001

Volume

107

Issue

2

Start / End Page

209 / 221

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Two-Hybrid System Techniques
  • Tissue Distribution
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Roundabout Proteins
  • Retroviridae
  • Receptors, Immunologic
  • RNA, Messenger
  • Protein Structure, Tertiary
 

Citation

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Wong, K., Ren, X. R., Huang, Y. Z., Xie, Y., Liu, G., Saito, H., … Rao, Y. (2001). Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell, 107(2), 209–221. https://doi.org/10.1016/s0092-8674(01)00530-x
Wong, K., X. R. Ren, Y. Z. Huang, Y. Xie, G. Liu, H. Saito, H. Tang, et al. “Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.Cell 107, no. 2 (October 19, 2001): 209–21. https://doi.org/10.1016/s0092-8674(01)00530-x.
Wong K, Ren XR, Huang YZ, Xie Y, Liu G, Saito H, et al. Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell. 2001 Oct 19;107(2):209–21.
Wong, K., et al. “Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.Cell, vol. 107, no. 2, Oct. 2001, pp. 209–21. Pubmed, doi:10.1016/s0092-8674(01)00530-x.
Wong K, Ren XR, Huang YZ, Xie Y, Liu G, Saito H, Tang H, Wen L, Brady-Kalnay SM, Mei L, Wu JY, Xiong WC, Rao Y. Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway. Cell. 2001 Oct 19;107(2):209–221.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

October 19, 2001

Volume

107

Issue

2

Start / End Page

209 / 221

Location

United States

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Two-Hybrid System Techniques
  • Tissue Distribution
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Roundabout Proteins
  • Retroviridae
  • Receptors, Immunologic
  • RNA, Messenger
  • Protein Structure, Tertiary