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Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf.

Publication ,  Journal Article
Lanigan, TM; Liu, A; Huang, YZ; Mei, L; Margolis, B; Guan, K-L
Published in: FASEB J
November 2003

Connector enhancer of KSR (CNK) is a multidomain protein that participates in Ras signaling in Drosophila eye development. In this report we identify the human homologue of CNK, termed CNK2A, and a truncated alternatively spliced variant, CNK2B. We characterize CNK2 phosphorylation, membrane localization, and interaction with Ras effector molecules. Our results show that MAPK signaling appears to play a role in the phosphorylation of CNK2 in vivo. CNK2 is found in both membrane and cytoplasmic fractions of the cell. In MDCK cells, full-length CNK2 is localized to the lateral plasma membrane. Consistent with previous reports, we show CNK2 interacts with Raf. CNK2 interaction was mapped to the regulatory and kinase domains of Raf, as well as to the carboxyl-terminal half of CNK2. CNK2 also interacts with the Ral signaling components, Ral GTPase, and the RalGDS family member Rlf. CNK2 interaction was mapped to the GEF domain of Rlf. The ability of CNK2 to interact with both Ras effector proteins Raf and Rlf suggests that CNK2 may integrate signals between MAPK and Ral pathways through a complex interplay of components.

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Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

November 2003

Volume

17

Issue

14

Start / End Page

2048 / 2060

Location

United States

Related Subject Headings

  • ral GTP-Binding Proteins
  • Transcription Factors
  • Sequence Homology, Amino Acid
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogene Proteins c-raf
  • Phosphorylation
  • Molecular Sequence Data
  • Membrane Proteins
  • MAP Kinase Signaling System
  • Humans
 

Citation

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MLA
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Lanigan, T. M., Liu, A., Huang, Y. Z., Mei, L., Margolis, B., & Guan, K.-L. (2003). Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf. FASEB J, 17(14), 2048–2060. https://doi.org/10.1096/fj.02-1096com
Lanigan, Thomas M., Albert Liu, Yang Z. Huang, Lin Mei, Ben Margolis, and Kun-Liang Guan. “Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf.FASEB J 17, no. 14 (November 2003): 2048–60. https://doi.org/10.1096/fj.02-1096com.
Lanigan TM, Liu A, Huang YZ, Mei L, Margolis B, Guan K-L. Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf. FASEB J. 2003 Nov;17(14):2048–60.
Lanigan, Thomas M., et al. “Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf.FASEB J, vol. 17, no. 14, Nov. 2003, pp. 2048–60. Pubmed, doi:10.1096/fj.02-1096com.
Lanigan TM, Liu A, Huang YZ, Mei L, Margolis B, Guan K-L. Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf. FASEB J. 2003 Nov;17(14):2048–2060.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

November 2003

Volume

17

Issue

14

Start / End Page

2048 / 2060

Location

United States

Related Subject Headings

  • ral GTP-Binding Proteins
  • Transcription Factors
  • Sequence Homology, Amino Acid
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogene Proteins c-raf
  • Phosphorylation
  • Molecular Sequence Data
  • Membrane Proteins
  • MAP Kinase Signaling System
  • Humans