Skip to main content
Journal cover image

The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.

Publication ,  Journal Article
Wiederrecht, G; Shuey, DJ; Kibbe, WA; Parker, CS
Published in: Cell
February 13, 1987

The heat shock transcription factor (HSTF) has been purified to apparent homogeneity from S. cerevisiae and D. melanogaster by sequence-specific DNA-affinity chromatography. A synthetic oligonucleotide containing an hsp83-like heat shock element (HSE) was prepared and ligated into concatamers and covalently coupled to Sepharose. This DNA-affinity resin allowed the rapid isolation of a yeast and a Drosophila protein with the same apparent molecular weight (70 kd). The yeast HSTF will bind to both its own and the Drosophila HSEs. Similarly, the Drosophila HSTF will bind to both its own and the yeast HSEs. The yeast and Drosophila HSTFs were subjected to preparative SDS gel electrophoresis, and the 70 kd polypeptides were eluted, renatured, and observed to generate the identical footprint pattern as the native HSTFs. Affinity-purified Drosophila HSTF was further shown to stimulate specific HSE-dependent transcription from a Drosophila hsp70 gene in vitro.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

February 13, 1987

Volume

48

Issue

3

Start / End Page

507 / 515

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae
  • Heat-Shock Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Drosophila melanogaster
  • Developmental Biology
  • DNA, Fungal
  • DNA
  • Chromatography, Affinity
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wiederrecht, G., Shuey, D. J., Kibbe, W. A., & Parker, C. S. (1987). The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties. Cell, 48(3), 507–515. https://doi.org/10.1016/0092-8674(87)90201-7
Wiederrecht, G., D. J. Shuey, W. A. Kibbe, and C. S. Parker. “The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.Cell 48, no. 3 (February 13, 1987): 507–15. https://doi.org/10.1016/0092-8674(87)90201-7.
Wiederrecht G, Shuey DJ, Kibbe WA, Parker CS. The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties. Cell. 1987 Feb 13;48(3):507–15.
Wiederrecht, G., et al. “The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.Cell, vol. 48, no. 3, Feb. 1987, pp. 507–15. Pubmed, doi:10.1016/0092-8674(87)90201-7.
Wiederrecht G, Shuey DJ, Kibbe WA, Parker CS. The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties. Cell. 1987 Feb 13;48(3):507–515.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

February 13, 1987

Volume

48

Issue

3

Start / End Page

507 / 515

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae
  • Heat-Shock Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Drosophila melanogaster
  • Developmental Biology
  • DNA, Fungal
  • DNA
  • Chromatography, Affinity