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Plasmonic nanochip for SERS chemical and biomedical sensing

Publication ,  Conference
Ngo, HT; Strobbia, P; Wang, HN; Crawford, BM; Fales, AM; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2017

The development of rapid, easy-to-use and highly sensitive DNA detection methods has received increasing interest for medical diagnostics and research purposes. Our laboratory has developed several chip-based DNA biosensors including molecular sentinel-on-chip (MSC), multiplex MSC, and inverse molecular sentinel-on-chip (iMS-on-Chip). These sensors use surface-enhanced Raman scattering (SERS) plasmonic chips, functionalized with DNA probes for single-step DNA detection. The sensing mechanisms is based on the hybridization of target sequences and DNA probes, resulting in a displacement of a SERS reporter from the chip surface. This distance increase results in change in SERS signal intensity from the reporter, thus indicating the capture, and therefore the presence, of the target nucleic acid sequence. The nucleic acid probes and the SERS chip, which compose the sensing platform, were designed for single-step DNA detection. The target sequences are detected by delivery of a sample solutions on a functionalized chip and characterization of the SERS signals, after 1-2 hr incubation. These techniques avoid labeling of the target sequence or washing to remove unreacted components, making them easy-to-use and cost effective. The use of SERS chip for medical diagnostics was demonstrated by detecting genetic biomarkers for respiratory viral infection and the DNA of dengue virus 4.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2017

Volume

10215

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Ngo, H. T., Strobbia, P., Wang, H. N., Crawford, B. M., Fales, A. M., & Vo-Dinh, T. (2017). Plasmonic nanochip for SERS chemical and biomedical sensing. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 10215). https://doi.org/10.1117/12.2267759
Ngo, H. T., P. Strobbia, H. N. Wang, B. M. Crawford, A. M. Fales, and T. Vo-Dinh. “Plasmonic nanochip for SERS chemical and biomedical sensing.” In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 10215, 2017. https://doi.org/10.1117/12.2267759.
Ngo HT, Strobbia P, Wang HN, Crawford BM, Fales AM, Vo-Dinh T. Plasmonic nanochip for SERS chemical and biomedical sensing. In: Proceedings of SPIE - The International Society for Optical Engineering. 2017.
Ngo, H. T., et al. “Plasmonic nanochip for SERS chemical and biomedical sensing.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 10215, 2017. Scopus, doi:10.1117/12.2267759.
Ngo HT, Strobbia P, Wang HN, Crawford BM, Fales AM, Vo-Dinh T. Plasmonic nanochip for SERS chemical and biomedical sensing. Proceedings of SPIE - The International Society for Optical Engineering. 2017.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2017

Volume

10215

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering