Skip to main content

The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines

Publication ,  Journal Article
Sun, T; Wang, Q; Balk, S; Brown, M; Lee, G-SM; Kantoff, P
Published in: Cancer Research
April 15, 2009

Androgen-dependent prostate cancer typically progresses to castration-resistant prostate cancer (CRPC) after the androgen deprivation therapy. MicroRNAs (miR) are noncoding small RNAs (19-25nt) that play an important role in the regulation of gene expression. Recent studies have shown that miR expression patterns are significantly different in normal and neoplastic prostate epithelial cells. However, the importance of miRs in the development of CRPC has not yet been explored. By performing genome-wide expression profiling of miRs, we found that expression levels of several miRs, in particular miR-221 and miR-222, were significantly increased in CRPC cells (the LNCaP-derived cell line LNCaP-Abl), compared with those in the androgen-dependent prostate cancer cell line (LNCaP). Overexpression of miR-221 or miR-222 in LNCaP or another androgen-dependent cell line, LAPC-4, significantly reduced the level of the dihydrotestosterone (DHT) induced up-regulation of prostate-specific antigen (PSA) expression and increased androgen-independent growth of LNCaP cells. Knocking down the expression level of miR-221 and miR-222 with antagonist miRs in the LNCaP-Abl cell line restored the response to the DHT induction of PSA transcription and also increased the growth response of the LNCaP-Abl cells to the androgen treatment. Changing the expression level of p27/kip1, a known target of miR-221 and miR-222, alone in LNCaP cells affected the DHT-independent cell growth but did not significantly influence the response of PSA transcription to the DHT treatment. In conclusion, our data suggest the involvement of miR-221 and miR-222 in the development or maintenance of the CRPC phenotype. [Cancer Res 2009;69(8):3356–63]

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

April 15, 2009

Volume

69

Issue

8

Start / End Page

3356 / 3363

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sun, T., Wang, Q., Balk, S., Brown, M., Lee, G.-S., & Kantoff, P. (2009). The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines. Cancer Research, 69(8), 3356–3363. https://doi.org/10.1158/0008-5472.can-08-4112
Sun, Tong, Qianben Wang, Steven Balk, Myles Brown, Gwo-Shu Mary Lee, and Philip Kantoff. “The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines.” Cancer Research 69, no. 8 (April 15, 2009): 3356–63. https://doi.org/10.1158/0008-5472.can-08-4112.
Sun T, Wang Q, Balk S, Brown M, Lee G-SM, Kantoff P. The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines. Cancer Research. 2009 Apr 15;69(8):3356–63.
Sun, Tong, et al. “The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines.” Cancer Research, vol. 69, no. 8, American Association for Cancer Research (AACR), Apr. 2009, pp. 3356–63. Crossref, doi:10.1158/0008-5472.can-08-4112.
Sun T, Wang Q, Balk S, Brown M, Lee G-SM, Kantoff P. The Role of microRNA-221 and microRNA-222 in Androgen-Independent Prostate Cancer Cell Lines. Cancer Research. American Association for Cancer Research (AACR); 2009 Apr 15;69(8):3356–3363.

Published In

Cancer Research

DOI

EISSN

1538-7445

ISSN

0008-5472

Publication Date

April 15, 2009

Volume

69

Issue

8

Start / End Page

3356 / 3363

Publisher

American Association for Cancer Research (AACR)

Related Subject Headings

  • Oncology & Carcinogenesis
  • 3211 Oncology and carcinogenesis
  • 3101 Biochemistry and cell biology
  • 1112 Oncology and Carcinogenesis