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Characterization of a chemotactic and cytotoxic proteinase from human skin.

Publication ,  Journal Article
Hatcher, VB; Lazarus, GS; Levine, N; Burk, PG; Yost, FJ
Published in: Biochim Biophys Acta
July 8, 1977
Published version (DOI) Link to item

A proteinase (EC 3.4.-.-) active at physiological pH has been isolated from human skin utilizing gel filtration and affinity chromatography techniques. The proteinase has a molecular weight of approx. 28 000 and it is inhibited by alpha 2-macroglobulin, alpha 1-antitrypsin, C-1 inactivatory, soybean trypsin inhibitor and diisopropyl fluorophosphate. 2njection of 1 ng of purified proteinase into rabbit skin induces polymorphonuclear leukocyte infiltration of the cutis. Inhibition of enzyme activity with diisopropyl fluorophosphate inhibits the chemotactic effect. Addition of 0.2 microgram/ml of purified proteinase to fibroblast cultures kills the cells within minutes. This proteinase may play a significant role in modulating the inflammatory response after cellular injury.

Duke Scholars

Gerald Sylvan Lazarus
Author Gerald Sylvan Lazarus Dermatology

Published In

Biochim Biophys Acta

DOI

10.1016/0005-2744(77)90018-3

ISSN

0006-3002

Publication Date

July 8, 1977

Volume

483

Issue

1

Start / End Page

160 / 171

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin Inhibitors
  • Skin
  • Rabbits
  • Protein Biosynthesis
  • Protease Inhibitors
  • Peptide Hydrolases
  • Molecular Weight
  • Leucine
  • Isoflurophate
 

Citation

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Hatcher, V. B., Lazarus, G. S., Levine, N., Burk, P. G., & Yost, F. J. (1977). Characterization of a chemotactic and cytotoxic proteinase from human skin. Biochim Biophys Acta, 483(1), 160–171. https://doi.org/10.1016/0005-2744(77)90018-3
Hatcher, V. B., G. S. Lazarus, N. Levine, P. G. Burk, and F. J. Yost. “Characterization of a chemotactic and cytotoxic proteinase from human skin.” Biochim Biophys Acta 483, no. 1 (July 8, 1977): 160–71. https://doi.org/10.1016/0005-2744(77)90018-3.
Hatcher VB, Lazarus GS, Levine N, Burk PG, Yost FJ. Characterization of a chemotactic and cytotoxic proteinase from human skin. Biochim Biophys Acta. 1977 Jul 8;483(1):160–71.
Hatcher, V. B., et al. “Characterization of a chemotactic and cytotoxic proteinase from human skin.” Biochim Biophys Acta, vol. 483, no. 1, July 1977, pp. 160–71. Pubmed, doi:10.1016/0005-2744(77)90018-3.
Hatcher VB, Lazarus GS, Levine N, Burk PG, Yost FJ. Characterization of a chemotactic and cytotoxic proteinase from human skin. Biochim Biophys Acta. 1977 Jul 8;483(1):160–171.

Published In

Biochim Biophys Acta

DOI

10.1016/0005-2744(77)90018-3

ISSN

0006-3002

Publication Date

July 8, 1977

Volume

483

Issue

1

Start / End Page

160 / 171

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin Inhibitors
  • Skin
  • Rabbits
  • Protein Biosynthesis
  • Protease Inhibitors
  • Peptide Hydrolases
  • Molecular Weight
  • Leucine
  • Isoflurophate