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Ovarian effects of a high lactose diet in the female rat.

Publication ,  Journal Article
Liu, G; Shi, F; Blas-Machado, U; Duong, Q; Davis, VL; Foster, WG; Hughes, CL
Published in: Reprod Nutr Dev
2005

Young women with galactosemia experience ovarian failure at a very early age raising concern about the ovarian toxicity of galactose. While galactose may be present in the diet as a monosaccharide, it is predominantly derived from cleavage of the disaccharide lactose within the intestine. Our previous studies in animals have shown that high galactose diets inhibit ovarian follicular development and long-term exposure to high lactose diets retards growth of rats. The objective of the present study was to determine whether galactose exposure in the form of dietary lactose mimics the effects found previously with diets rich in galactose. Sixty female Long-Evans rats (25-day-old) were randomly assigned to two groups and fed a control diet (41.9% glucose in AIN93G [American Institute of Nutrition], CON) before lactose treatment. Unilateral ovariectomy (uOVX) was performed on half of the rats in each group to determine baseline ovarian follicle numbers. The study diet was a high lactose diet (HLD) containing 41.9% lactose in AIN93G. Study diet exposure started 1 month after uOVX (3 months old) and continued for 7 months in the treatment group. The control group remained on the 41.9% glucose diet throughout. Vaginal cytology, ovarian morphometric analyses, and serum concentrations of estradiol and progesterone were examined. Long-term exposure to the HLD decreased the body weights of animals and progesterone concentrations in the serum but produced no harmful effects on ovarian morphology or function. Beginning at 5 months of age (two months of lactose treatment) increasing numbers of females began to cycle irregularly but there was no difference between the glucose and lactose diet groups. These negative findings imply that administration of galactose in the form of lactose seems to be much less toxic than when galactose is fed to animals. From a human health perspective, these results are somewhat reassuring, since in general, women eat lactose-containing foods rather than foods that contain large amounts of free galactose.

Duke Scholars

Published In

Reprod Nutr Dev

DOI

ISSN

0926-5287

Publication Date

2005

Volume

45

Issue

2

Start / End Page

185 / 192

Location

France

Related Subject Headings

  • Vagina
  • Rats, Long-Evans
  • Rats
  • Random Allocation
  • Progesterone
  • Primary Ovarian Insufficiency
  • Ovarian Follicle
  • Models, Animal
  • Lactose
  • Infertility
 

Citation

APA
Chicago
ICMJE
MLA
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Liu, G., Shi, F., Blas-Machado, U., Duong, Q., Davis, V. L., Foster, W. G., & Hughes, C. L. (2005). Ovarian effects of a high lactose diet in the female rat. Reprod Nutr Dev, 45(2), 185–192. https://doi.org/10.1051/rnd:2005010
Liu, Gentao, Fangxiong Shi, Uriel Blas-Machado, Quynh Duong, Vicki L. Davis, Warren G. Foster, and Claude L. Hughes. “Ovarian effects of a high lactose diet in the female rat.Reprod Nutr Dev 45, no. 2 (2005): 185–92. https://doi.org/10.1051/rnd:2005010.
Liu G, Shi F, Blas-Machado U, Duong Q, Davis VL, Foster WG, et al. Ovarian effects of a high lactose diet in the female rat. Reprod Nutr Dev. 2005;45(2):185–92.
Liu, Gentao, et al. “Ovarian effects of a high lactose diet in the female rat.Reprod Nutr Dev, vol. 45, no. 2, 2005, pp. 185–92. Pubmed, doi:10.1051/rnd:2005010.
Liu G, Shi F, Blas-Machado U, Duong Q, Davis VL, Foster WG, Hughes CL. Ovarian effects of a high lactose diet in the female rat. Reprod Nutr Dev. 2005;45(2):185–192.
Journal cover image

Published In

Reprod Nutr Dev

DOI

ISSN

0926-5287

Publication Date

2005

Volume

45

Issue

2

Start / End Page

185 / 192

Location

France

Related Subject Headings

  • Vagina
  • Rats, Long-Evans
  • Rats
  • Random Allocation
  • Progesterone
  • Primary Ovarian Insufficiency
  • Ovarian Follicle
  • Models, Animal
  • Lactose
  • Infertility