Flow Cytometry for the Immunotoxicologist.
Publication
, Journal Article
Espenschied, ST; Tighe, RM; Gowdy, KM
Published in: Methods Mol Biol
2018
Assessing the immunotoxicity of xenobiotics by current regulatory testing has revealed compounds that can cause immunosuppression and stimulation. Flow cytometry is a cutting edge technique that can provide data on how toxicants can alter the quality and quantity of the immune response after exposure. Here we describe protocols for how to use flow cytometry to measure the immune response in multiple rodent organs (blood and lymphoid and nonlymphoid) as well as in novel models recently being utilized in the field of toxicology. These methods can be used for current testing and to determine mechanisms by which a xenobiotic can cause immunotoxicity.
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1803
Start / End Page
183 / 197
Location
United States
Related Subject Headings
- Zebrafish
- Toxicology
- Toxicity Tests
- Mice
- Lymphoid Tissue
- Immune System
- Humans
- Flow Cytometry
- Developmental Biology
- Animals, Genetically Modified
Citation
APA
Chicago
ICMJE
MLA
NLM
Espenschied, S. T., Tighe, R. M., & Gowdy, K. M. (2018). Flow Cytometry for the Immunotoxicologist. Methods Mol Biol, 1803, 183–197. https://doi.org/10.1007/978-1-4939-8549-4_12
Espenschied, Scott T., Robert M. Tighe, and Kymberly M. Gowdy. “Flow Cytometry for the Immunotoxicologist.” Methods Mol Biol 1803 (2018): 183–97. https://doi.org/10.1007/978-1-4939-8549-4_12.
Espenschied ST, Tighe RM, Gowdy KM. Flow Cytometry for the Immunotoxicologist. Methods Mol Biol. 2018;1803:183–97.
Espenschied, Scott T., et al. “Flow Cytometry for the Immunotoxicologist.” Methods Mol Biol, vol. 1803, 2018, pp. 183–97. Pubmed, doi:10.1007/978-1-4939-8549-4_12.
Espenschied ST, Tighe RM, Gowdy KM. Flow Cytometry for the Immunotoxicologist. Methods Mol Biol. 2018;1803:183–197.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1803
Start / End Page
183 / 197
Location
United States
Related Subject Headings
- Zebrafish
- Toxicology
- Toxicity Tests
- Mice
- Lymphoid Tissue
- Immune System
- Humans
- Flow Cytometry
- Developmental Biology
- Animals, Genetically Modified