Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro.
Publication
, Journal Article
Zhou, J; Sinha, RA; Lesmana, R; Yau, WWY; Yen, PM
Published in: Methods Mol Biol
2018
Autophagy is an evolutionarily conserved intracellular catabolic process that is essential for cellular housekeeping and nutrient homeostasis. Recently, we provided evidence that thyroid hormone (TH) is a major inducer of autophagy in mammalian cells. Here, we describe a method for detecting TH-induced autophagic flux in hepatic, muscle, and brown adipocyte cells using lysosomal inhibitor bafilomycin A1 (BafA1) and conventional Western blot techniques.
Duke Scholars
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1801
Start / End Page
111 / 122
Location
United States
Related Subject Headings
- Thyroid Hormones
- Receptors, Thyroid Hormone
- Protein Isoforms
- Myoblasts
- Microtubule-Associated Proteins
- Macrolides
- Lysosomes
- Humans
- Developmental Biology
- Cells, Cultured
Citation
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Chicago
ICMJE
MLA
NLM
Zhou, J., Sinha, R. A., Lesmana, R., Yau, W. W. Y., & Yen, P. M. (2018). Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro. Methods Mol Biol, 1801, 111–122. https://doi.org/10.1007/978-1-4939-7902-8_11
Zhou, Jin, Rohit A. Sinha, Ronny Lesmana, Winifred W. Y. Yau, and Paul M. Yen. “Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro.” Methods Mol Biol 1801 (2018): 111–22. https://doi.org/10.1007/978-1-4939-7902-8_11.
Zhou J, Sinha RA, Lesmana R, Yau WWY, Yen PM. Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro. Methods Mol Biol. 2018;1801:111–22.
Zhou, Jin, et al. “Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro.” Methods Mol Biol, vol. 1801, 2018, pp. 111–22. Pubmed, doi:10.1007/978-1-4939-7902-8_11.
Zhou J, Sinha RA, Lesmana R, Yau WWY, Yen PM. Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro. Methods Mol Biol. 2018;1801:111–122.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1801
Start / End Page
111 / 122
Location
United States
Related Subject Headings
- Thyroid Hormones
- Receptors, Thyroid Hormone
- Protein Isoforms
- Myoblasts
- Microtubule-Associated Proteins
- Macrolides
- Lysosomes
- Humans
- Developmental Biology
- Cells, Cultured