Scholars@Duke publication: Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.

Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.

Publication , Journal Article

Stephenson, EC; Erba, HP; Gall, JG

Published in: Nucleic Acids Res

May 25, 1981

We report the cloning and characterization of a histone gene cluster of the newt Notophthalamus viridescens. Fragments containing newt histone genes were identified in whole genome Southern blots; these fragments were cloned into a bacteriophage lambda cloning vector constructed for this purpose. The positions of most of the histone genes were determined by hybridizing subcloned sea urchin histone genes to digests of the cloned newt gene cluster. The position of each gene was verified, and its polarity determined by sequencing a portion of each. The order of the genes in the cloned segment is H1-H3-H2B-H2A-H4, with each of the genes but H2B being transcribed in the same direction. Subcloned segments of the histone gene repeat were used to determine the size of each newt oocyte histone mRNA.

Duke Scholars

Author Harry Paul Erba Medicine, Hematologic Malignancies and Cellular Therapy

Published In

Nucleic Acids Res

DOI

10.1093/nar/9.10.2281

ISSN

0305-1048

Publication Date

May 25, 1981

Volume

Issue

Start / End Page

2281 / 2295

Location

England

Related Subject Headings

Salamandridae
Plasmids
Nucleic Acid Hybridization
Histones
Genes
Developmental Biology
DNA, Recombinant
DNA Restriction Enzymes
Cloning, Molecular
Base Sequence

Citation

APA

Chicago

ICMJE

MLA

NLM

Stephenson, E. C., Erba, H. P., & Gall, J. G. (1981). Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens. Nucleic Acids Res, 9(10), 2281–2295. https://doi.org/10.1093/nar/9.10.2281

Stephenson, E. C., H. P. Erba, and J. G. Gall. “Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.” Nucleic Acids Res 9, no. 10 (May 25, 1981): 2281–95. https://doi.org/10.1093/nar/9.10.2281.

Stephenson EC, Erba HP, Gall JG. Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens. Nucleic Acids Res. 1981 May 25;9(10):2281–95.

Stephenson, E. C., et al. “Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens.” Nucleic Acids Res, vol. 9, no. 10, May 1981, pp. 2281–95. Pubmed, doi:10.1093/nar/9.10.2281.

Stephenson EC, Erba HP, Gall JG. Characterization of a cloned histone gene cluster of the newt Notophthalamus viridescens. Nucleic Acids Res. 1981 May 25;9(10):2281–2295.

Published In

Nucleic Acids Res

DOI

10.1093/nar/9.10.2281

ISSN

0305-1048

Publication Date

May 25, 1981

Volume

Issue

Start / End Page

2281 / 2295

Location

England

Related Subject Headings

Salamandridae
Plasmids
Nucleic Acid Hybridization
Histones
Genes
Developmental Biology
DNA, Recombinant
DNA Restriction Enzymes
Cloning, Molecular
Base Sequence