Isolation and Characterization of Human Lung Myeloid Cells.
Publication
, Journal Article
Yu, Y-RA; Tighe, RM
Published in: Methods Mol Biol
2018
Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.
Duke Scholars
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1809
Start / End Page
111 / 119
Location
United States
Related Subject Headings
- Myeloid Cells
- Monocytes
- Macrophages, Alveolar
- Lung
- Immunophenotyping
- Humans
- Flow Cytometry
- Developmental Biology
- Cell Survival
- Cell Separation
Citation
APA
Chicago
ICMJE
MLA
NLM
Yu, Y.-R., & Tighe, R. M. (2018). Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol, 1809, 111–119. https://doi.org/10.1007/978-1-4939-8570-8_9
Yu, Yen-Rei A., and Robert M. Tighe. “Isolation and Characterization of Human Lung Myeloid Cells.” Methods Mol Biol 1809 (2018): 111–19. https://doi.org/10.1007/978-1-4939-8570-8_9.
Yu Y-RA, Tighe RM. Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol. 2018;1809:111–9.
Yu, Yen-Rei A., and Robert M. Tighe. “Isolation and Characterization of Human Lung Myeloid Cells.” Methods Mol Biol, vol. 1809, 2018, pp. 111–19. Pubmed, doi:10.1007/978-1-4939-8570-8_9.
Yu Y-RA, Tighe RM. Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol. 2018;1809:111–119.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1809
Start / End Page
111 / 119
Location
United States
Related Subject Headings
- Myeloid Cells
- Monocytes
- Macrophages, Alveolar
- Lung
- Immunophenotyping
- Humans
- Flow Cytometry
- Developmental Biology
- Cell Survival
- Cell Separation