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Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.

Publication ,  Journal Article
Muroyama, A; Terwilliger, M; Dong, B; Suh, H; Lechler, T
Published in: Mol Biol Cell
July 1, 2018

In most differentiated cells, microtubules reorganize into noncentrosomal arrays that are cell-type specific. In the columnar absorptive enterocytes of the intestine, microtubules form polarized apical-basal arrays that have been proposed to play multiple roles. However, in vivo testing of these hypotheses has been hampered by a lack of genetic tools to specifically perturb microtubules. Here we analyze mice in which microtubules are disrupted by conditional inducible expression of the microtubule-severing protein spastin. Spastin overexpression resulted in multiple cellular defects, including aberrations in nuclear and organelle positioning and deficient nutrient transport. However, cell shape, adhesion, and polarity remained intact, and mutant mice continued to thrive. Notably, the phenotypes of microtubule disruption are similar to those induced by microtubule disorganization upon loss of CAMSAP3/Nezha. These data demonstrate that enterocyte microtubules have important roles in organelle organization but are not essential for growth under homeostatic conditions.

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Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

July 1, 2018

Volume

29

Issue

13

Start / End Page

1533 / 1541

Location

United States

Related Subject Headings

  • Microtubules
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Intracellular Space
  • Intestines
  • Intestinal Mucosa
  • Enterocytes
  • Developmental Biology
  • Centrosome
  • Cell Differentiation
 

Citation

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Muroyama, A., Terwilliger, M., Dong, B., Suh, H., & Lechler, T. (2018). Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport. Mol Biol Cell, 29(13), 1533–1541. https://doi.org/10.1091/mbc.E18-01-0057
Muroyama, Andrew, Michael Terwilliger, Bushu Dong, Harrison Suh, and Terry Lechler. “Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Mol Biol Cell 29, no. 13 (July 1, 2018): 1533–41. https://doi.org/10.1091/mbc.E18-01-0057.
Muroyama A, Terwilliger M, Dong B, Suh H, Lechler T. Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport. Mol Biol Cell. 2018 Jul 1;29(13):1533–41.
Muroyama, Andrew, et al. “Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Mol Biol Cell, vol. 29, no. 13, July 2018, pp. 1533–41. Pubmed, doi:10.1091/mbc.E18-01-0057.
Muroyama A, Terwilliger M, Dong B, Suh H, Lechler T. Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport. Mol Biol Cell. 2018 Jul 1;29(13):1533–1541.

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

July 1, 2018

Volume

29

Issue

13

Start / End Page

1533 / 1541

Location

United States

Related Subject Headings

  • Microtubules
  • Microtubule-Associated Proteins
  • Mice, Knockout
  • Intracellular Space
  • Intestines
  • Intestinal Mucosa
  • Enterocytes
  • Developmental Biology
  • Centrosome
  • Cell Differentiation