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Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease.

Publication ,  Journal Article
Barisoni, L; Jennette, JC; Colvin, R; Sitaraman, S; Bragat, A; Castelli, J; Walker, D; Boudes, P
Published in: Arch Pathol Lab Med
July 2012

CONTEXT: Assessing the amount of globotriaosylceramide inclusions in renal peritubular capillaries by a semiquantitative approach is a standard and useful measure of therapeutic efficacy in Fabry disease, achievable by light microscopy analysis. OBJECTIVE: To describe a novel virtual microscopy quantitative method to measure globotriaosylceramide inclusions (Barisoni Lipid Inclusion Scoring System [BLISS]) in renal biopsies from patients with Fabry disease. DESIGN: Plastic embedded 1-µm-thick sections from kidney biopsies from 17 patients enrolled in a Fabry disease clinical trial were evaluated using a standard semiquantitative methodology and BLISS to compare sensitivity. We also tested intrareader and interreader variability of BLISS and compared results from conventional light microscopy analysis with a virtual microscopy-based methodology. Peritubular capillaries were first annotated on digital images of whole slides by 1 pathologist and then scored for globotriaosylceramide inclusions by 2 additional pathologists. RESULTS: We demonstrated that (1) quantitative analysis by BLISS results in detection of small amount of globotriaosylceramide inclusions even when by semiquantitative analysis the score is 0, (2) application of BLISS combined with conventional light microscopy results in low intrareader and interreader variability, and (3) BLISS combined with virtual microscopy results in significant reduction of intrareader and interreader variability compared with BLISS-light microscopy. CONCLUSIONS: BLISS is a simpler and more sensitive scoring system compared to the semiquantitative approach. The virtual microscopy-based methodology increases accuracy and reproducibility; moreover, it provides a permanent record of retrievable data with full transparency in clinical trials.

Duke Scholars

Published In

Arch Pathol Lab Med

DOI

EISSN

1543-2165

Publication Date

July 2012

Volume

136

Issue

7

Start / End Page

816 / 824

Location

United States

Related Subject Headings

  • Trihexosylceramides
  • Pathology
  • Microscopy
  • Male
  • Kidney
  • Humans
  • Female
  • Fabry Disease
  • Capillaries
  • 3202 Clinical sciences
 

Citation

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ICMJE
MLA
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Barisoni, L., Jennette, J. C., Colvin, R., Sitaraman, S., Bragat, A., Castelli, J., … Boudes, P. (2012). Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease. Arch Pathol Lab Med, 136(7), 816–824. https://doi.org/10.5858/arpa.2011-0350-OA
Barisoni, Laura, J Charles Jennette, Robert Colvin, Sheela Sitaraman, Alexander Bragat, Jeff Castelli, Dan Walker, and Pol Boudes. “Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease.Arch Pathol Lab Med 136, no. 7 (July 2012): 816–24. https://doi.org/10.5858/arpa.2011-0350-OA.
Barisoni L, Jennette JC, Colvin R, Sitaraman S, Bragat A, Castelli J, et al. Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease. Arch Pathol Lab Med. 2012 Jul;136(7):816–24.
Barisoni, Laura, et al. “Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease.Arch Pathol Lab Med, vol. 136, no. 7, July 2012, pp. 816–24. Pubmed, doi:10.5858/arpa.2011-0350-OA.
Barisoni L, Jennette JC, Colvin R, Sitaraman S, Bragat A, Castelli J, Walker D, Boudes P. Novel quantitative method to evaluate globotriaosylceramide inclusions in renal peritubular capillaries by virtual microscopy in patients with fabry disease. Arch Pathol Lab Med. 2012 Jul;136(7):816–824.

Published In

Arch Pathol Lab Med

DOI

EISSN

1543-2165

Publication Date

July 2012

Volume

136

Issue

7

Start / End Page

816 / 824

Location

United States

Related Subject Headings

  • Trihexosylceramides
  • Pathology
  • Microscopy
  • Male
  • Kidney
  • Humans
  • Female
  • Fabry Disease
  • Capillaries
  • 3202 Clinical sciences