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Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.

Publication ,  Journal Article
Yo, Y; Braun, MC; Barisoni, L; Mobaraki, H; Lu, H; Shrivastav, S; Owens, J; Kopp, JB
Published in: Nephron. Experimental nephrology
January 2003

In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation.

Duke Scholars

Published In

Nephron. Experimental nephrology

DOI

EISSN

1660-2129

ISSN

1660-8151

Publication Date

January 2003

Volume

93

Issue

3

Start / End Page

e92 / 106

Related Subject Headings

  • Urology & Nephrology
  • Time Factors
  • Sheep
  • Organ Specificity
  • Mice, SCID
  • Mice, Inbred Strains
  • Mice, Inbred BALB C
  • Mice, Congenic
  • Mice
  • Lung
 

Citation

APA
Chicago
ICMJE
MLA
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Yo, Y., Braun, M. C., Barisoni, L., Mobaraki, H., Lu, H., Shrivastav, S., … Kopp, J. B. (2003). Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice. Nephron. Experimental Nephrology, 93(3), e92-106. https://doi.org/10.1159/000069551
Yo, Yoshikage, Michael C. Braun, Laura Barisoni, Hideko Mobaraki, Huiyan Lu, Shashi Shrivastav, Jennie Owens, and Jeffrey B. Kopp. “Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.Nephron. Experimental Nephrology 93, no. 3 (January 2003): e92-106. https://doi.org/10.1159/000069551.
Yo Y, Braun MC, Barisoni L, Mobaraki H, Lu H, Shrivastav S, et al. Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice. Nephron Experimental nephrology. 2003 Jan;93(3):e92-106.
Yo, Yoshikage, et al. “Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.Nephron. Experimental Nephrology, vol. 93, no. 3, Jan. 2003, pp. e92-106. Epmc, doi:10.1159/000069551.
Yo Y, Braun MC, Barisoni L, Mobaraki H, Lu H, Shrivastav S, Owens J, Kopp JB. Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice. Nephron Experimental nephrology. 2003 Jan;93(3):e92-106.
Journal cover image

Published In

Nephron. Experimental nephrology

DOI

EISSN

1660-2129

ISSN

1660-8151

Publication Date

January 2003

Volume

93

Issue

3

Start / End Page

e92 / 106

Related Subject Headings

  • Urology & Nephrology
  • Time Factors
  • Sheep
  • Organ Specificity
  • Mice, SCID
  • Mice, Inbred Strains
  • Mice, Inbred BALB C
  • Mice, Congenic
  • Mice
  • Lung