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Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis.

Publication ,  Journal Article
Iba, T; Levy, JH; Hirota, T; Hiki, M; Sato, K; Murakami, T; Nagaoka, I
Published in: Thromb Res
November 2018

INTRODUCTION: Injury and loss of the endothelial glycocalyx occur during the early phase of sepsis. We previously showed that antithrombin has a protective effect on this structure in vitro. Here, we investigated the possible protective effects of antithrombin in an animal model of sepsis. METHODS: Wistar rats were injected with endotoxin, and circulating levels of syndecan-1, hyaluronan, albumin, lactate and other biomarkers were measured in an antithrombin-treated group and an untreated control group (n = 6 in each group). Intravital microscopy was used to observe leukocyte adhesion, microcirculation, and syndecan-1 staining. RESULTS: The circulating levels of syndecan-1 and hyaluronan were significantly reduced in the antithrombin-treated group, compared with the untreated controls. Lactate levels and albumin reduction were significantly attenuated in the antithrombin-treated group. Intravital microscopic observation revealed that both leukocyte adhesion and blood flow were better maintained in the treatment group. The syndecan-1 lining was disrupted after endotoxin treatment, and this derangement was attenuated by treatment with antithrombin. CONCLUSION: Antithrombin effectively maintained microcirculation and vascular integrity by protecting the glycocalyx in a rat sepsis model.

Duke Scholars

Published In

Thromb Res

DOI

EISSN

1879-2472

Publication Date

November 2018

Volume

171

Start / End Page

1 / 6

Location

United States

Related Subject Headings

  • Syndecan-1
  • Sepsis
  • Rats, Wistar
  • Microcirculation
  • Inflammation
  • Hyaluronic Acid
  • Glycocalyx
  • Endotoxins
  • Endothelium, Vascular
  • Cardiovascular System & Hematology
 

Citation

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Iba, T., Levy, J. H., Hirota, T., Hiki, M., Sato, K., Murakami, T., & Nagaoka, I. (2018). Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis. Thromb Res, 171, 1–6. https://doi.org/10.1016/j.thromres.2018.09.042
Iba, Toshiaki, Jerrold H. Levy, Tatsuhiko Hirota, Makoto Hiki, Koichi Sato, Taisuke Murakami, and Isao Nagaoka. “Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis.Thromb Res 171 (November 2018): 1–6. https://doi.org/10.1016/j.thromres.2018.09.042.
Iba T, Levy JH, Hirota T, Hiki M, Sato K, Murakami T, et al. Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis. Thromb Res. 2018 Nov;171:1–6.
Iba, Toshiaki, et al. “Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis.Thromb Res, vol. 171, Nov. 2018, pp. 1–6. Pubmed, doi:10.1016/j.thromres.2018.09.042.
Iba T, Levy JH, Hirota T, Hiki M, Sato K, Murakami T, Nagaoka I. Protection of the endothelial glycocalyx by antithrombin in an endotoxin-induced rat model of sepsis. Thromb Res. 2018 Nov;171:1–6.
Journal cover image

Published In

Thromb Res

DOI

EISSN

1879-2472

Publication Date

November 2018

Volume

171

Start / End Page

1 / 6

Location

United States

Related Subject Headings

  • Syndecan-1
  • Sepsis
  • Rats, Wistar
  • Microcirculation
  • Inflammation
  • Hyaluronic Acid
  • Glycocalyx
  • Endotoxins
  • Endothelium, Vascular
  • Cardiovascular System & Hematology