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Immunoinhibition of intracellular protein digestion in macrophages.

Publication ,  Journal Article
Dingle, JT; Poole, AR; Lazarus, GS; Barrett, AJ
Published in: J Exp Med
May 1, 1973

Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells remained viable, but became very vacuolated. Both sheep IgG and hemoglobin were demonstrated immunocytochemically in vacuoles most of which could also be shown to contain cathepsin D. When the antiserum was removed, cells regained their normal morphology, and digestion of endocytosed proteins returned to normal. These results indicate that cathepsin D can be inhibited within lysosomes of viable cells, in which it plays a major role in the intracellular digestion of certain proteins.

Duke Scholars

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1973

Volume

137

Issue

5

Start / End Page

1124 / 1141

Location

United States

Related Subject Headings

  • Tritium
  • Sulfur Isotopes
  • Sheep
  • Rabbits
  • Proteins
  • Microscopy, Electron
  • Macrophages
  • Lysosomes
  • Immunology
  • Immunoglobulin G
 

Citation

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Chicago
ICMJE
MLA
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Dingle, J. T., Poole, A. R., Lazarus, G. S., & Barrett, A. J. (1973). Immunoinhibition of intracellular protein digestion in macrophages. J Exp Med, 137(5), 1124–1141. https://doi.org/10.1084/jem.137.5.1124
Dingle, J. T., A. R. Poole, G. S. Lazarus, and A. J. Barrett. “Immunoinhibition of intracellular protein digestion in macrophages.J Exp Med 137, no. 5 (May 1, 1973): 1124–41. https://doi.org/10.1084/jem.137.5.1124.
Dingle JT, Poole AR, Lazarus GS, Barrett AJ. Immunoinhibition of intracellular protein digestion in macrophages. J Exp Med. 1973 May 1;137(5):1124–41.
Dingle, J. T., et al. “Immunoinhibition of intracellular protein digestion in macrophages.J Exp Med, vol. 137, no. 5, May 1973, pp. 1124–41. Pubmed, doi:10.1084/jem.137.5.1124.
Dingle JT, Poole AR, Lazarus GS, Barrett AJ. Immunoinhibition of intracellular protein digestion in macrophages. J Exp Med. 1973 May 1;137(5):1124–1141.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

May 1, 1973

Volume

137

Issue

5

Start / End Page

1124 / 1141

Location

United States

Related Subject Headings

  • Tritium
  • Sulfur Isotopes
  • Sheep
  • Rabbits
  • Proteins
  • Microscopy, Electron
  • Macrophages
  • Lysosomes
  • Immunology
  • Immunoglobulin G