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Degradation of collagen by a human granulocyte collagenolytic system.

Publication ,  Journal Article
Lazarus, GS; Daniels, JR; Brown, RS; Bladen, HA; Fullmer, HM
Published in: J Clin Invest
December 1968

This report suggests a mechanism for collagen degradation mediated by human granulocytic leukocytes. A specific collagenase, which is extractable from human granulocytes, has been partially purified by DEAE chromatography. This collagenolytic enzyme is operative at physiological pH and is inhibited by EDTA, cysteine, and reduced glutathione but not by human serum. The enzyme cleaves the collagen molecule into two specific products, without loss of helical conformation. Electron micrographs of segment long spacing aggregates indicate that the cleavage occurs one-quarter of the length from the carboxy terminal end of the molecule. Experiments with crude extracts from granulocytes suggest that the specific products of granulocyte collagenase activity are then degraded by other proteases present in the human granulocyte.

Duke Scholars

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

December 1968

Volume

47

Issue

12

Start / End Page

2622 / 2629

Location

United States

Related Subject Headings

  • Microscopy, Electron
  • Microbial Collagenase
  • Leukocytes
  • Immunology
  • Hydrogen-Ion Concentration
  • Humans
  • Hot Temperature
  • Glutathione
  • Electrophoresis
  • Edetic Acid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lazarus, G. S., Daniels, J. R., Brown, R. S., Bladen, H. A., & Fullmer, H. M. (1968). Degradation of collagen by a human granulocyte collagenolytic system. J Clin Invest, 47(12), 2622–2629. https://doi.org/10.1172/JCI105945
Lazarus, G. S., J. R. Daniels, R. S. Brown, H. A. Bladen, and H. M. Fullmer. “Degradation of collagen by a human granulocyte collagenolytic system.J Clin Invest 47, no. 12 (December 1968): 2622–29. https://doi.org/10.1172/JCI105945.
Lazarus GS, Daniels JR, Brown RS, Bladen HA, Fullmer HM. Degradation of collagen by a human granulocyte collagenolytic system. J Clin Invest. 1968 Dec;47(12):2622–9.
Lazarus, G. S., et al. “Degradation of collagen by a human granulocyte collagenolytic system.J Clin Invest, vol. 47, no. 12, Dec. 1968, pp. 2622–29. Pubmed, doi:10.1172/JCI105945.
Lazarus GS, Daniels JR, Brown RS, Bladen HA, Fullmer HM. Degradation of collagen by a human granulocyte collagenolytic system. J Clin Invest. 1968 Dec;47(12):2622–2629.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

December 1968

Volume

47

Issue

12

Start / End Page

2622 / 2629

Location

United States

Related Subject Headings

  • Microscopy, Electron
  • Microbial Collagenase
  • Leukocytes
  • Immunology
  • Hydrogen-Ion Concentration
  • Humans
  • Hot Temperature
  • Glutathione
  • Electrophoresis
  • Edetic Acid