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Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG.

Publication ,  Journal Article
Morioka, S; Lazarus, GS; Jensen, PJ
Published in: J Invest Dermatol
November 1987

Pemphigus IgG induces acantholysis in skin organ culture without the involvement of complement. Urokinase-type plasminogen activator, a proteolytic enzyme, has been implicated in the development of acantholysis. To test this hypothesis, we prepared a rabbit anti-urokinase antibody, which inhibited the plasminogen activator activity in normal human epidermis and in cultured keratinocytes. When added to skin organ cultures along with pemphigus IgG, anti-urokinase IgG completely prevented the development of acantholysis. Normal or preimmune rabbit IgG had no effect on pemphigus IgG-induced acantholysis. Plasminogen activator converts the zymogen plasminogen to its active form plasmin, a broad specificity serine proteinase. When high concentrations of plasminogen alone were added to skin organ culture, acantholysis of the pemphigus foliaceous type was induced. Anti-urokinase antibody also inhibited plasminogen-induced acantholysis. These results strongly support a pivotal role for plasminogen activator in the development of acantholysis.

Duke Scholars

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

November 1987

Volume

89

Issue

5

Start / End Page

474 / 477

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Skin Diseases
  • Rabbits
  • Plasminogen Activators
  • Pemphigus
  • Organ Culture Techniques
  • Male
  • Infant, Newborn
  • Immunoglobulin G
  • Humans
 

Citation

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ICMJE
MLA
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Morioka, S., Lazarus, G. S., & Jensen, P. J. (1987). Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG. J Invest Dermatol, 89(5), 474–477. https://doi.org/10.1111/1523-1747.ep12460937
Morioka, S., G. S. Lazarus, and P. J. Jensen. “Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG.J Invest Dermatol 89, no. 5 (November 1987): 474–77. https://doi.org/10.1111/1523-1747.ep12460937.
Morioka S, Lazarus GS, Jensen PJ. Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG. J Invest Dermatol. 1987 Nov;89(5):474–7.
Morioka, S., et al. “Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG.J Invest Dermatol, vol. 89, no. 5, Nov. 1987, pp. 474–77. Pubmed, doi:10.1111/1523-1747.ep12460937.
Morioka S, Lazarus GS, Jensen PJ. Involvement of urokinase-type plasminogen activator in acantholysis induced by pemphigus IgG. J Invest Dermatol. 1987 Nov;89(5):474–477.
Journal cover image

Published In

J Invest Dermatol

DOI

ISSN

0022-202X

Publication Date

November 1987

Volume

89

Issue

5

Start / End Page

474 / 477

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Skin Diseases
  • Rabbits
  • Plasminogen Activators
  • Pemphigus
  • Organ Culture Techniques
  • Male
  • Infant, Newborn
  • Immunoglobulin G
  • Humans