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Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.

Publication ,  Journal Article
Price, LB; Liu, CM; Melendez, JH; Frankel, YM; Engelthaler, D; Aziz, M; Bowers, J; Rattray, R; Ravel, J; Kingsley, C; Keim, PS; Lazarus, GS ...
Published in: PLoS One
July 31, 2009

BACKGROUND: Bacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes and antibiotics on chronic wound microbiota. METHODOLOGY/PRINCIPAL FINDINGS: We prospectively enrolled 24 patients at a referral wound center in Baltimore, MD; sampled patients' wounds by curette; cultured samples under aerobic and anaerobic conditions; and pyrosequenced the 16S rRNA V3 hypervariable region. The 16S rRNA gene-based analyses revealed an average of 10 different bacterial families in wounds--approximately 4 times more than estimated by culture-based analyses. Fastidious anaerobic bacteria belonging to the Clostridiales family XI were among the most prevalent bacteria identified exclusively by 16S rRNA gene-based analyses. Community-scale analyses showed that wound microbiota from antibiotic treated patients were significantly different from untreated patients (p = 0.007) and were characterized by increased Pseudomonadaceae abundance. These analyses also revealed that antibiotic use was associated with decreased Streptococcaceae among diabetics and that Streptococcaceae was more abundant among diabetics as compared to non-diabetics. CONCLUSIONS/SIGNIFICANCE: The 16S rRNA gene-based analyses revealed complex bacterial communities including anaerobic bacteria that may play causative roles in the non-healing state of some chronic wounds. Our data suggest that antimicrobial therapy alters community structure--reducing some bacteria while selecting for others.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

July 31, 2009

Volume

4

Issue

7

Start / End Page

e6462

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • RNA, Ribosomal, 16S
  • Middle Aged
  • Microbial Sensitivity Tests
  • Male
  • Humans
  • General Science & Technology
  • Female
  • Diabetes Mellitus
  • Cohort Studies
 

Citation

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Price, L. B., Liu, C. M., Melendez, J. H., Frankel, Y. M., Engelthaler, D., Aziz, M., … Zenilman, J. M. (2009). Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One, 4(7), e6462. https://doi.org/10.1371/journal.pone.0006462
Price, Lance B., Cindy M. Liu, Johan H. Melendez, Yelena M. Frankel, David Engelthaler, Maliha Aziz, Jolene Bowers, et al. “Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.PLoS One 4, no. 7 (July 31, 2009): e6462. https://doi.org/10.1371/journal.pone.0006462.
Price LB, Liu CM, Melendez JH, Frankel YM, Engelthaler D, Aziz M, et al. Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One. 2009 Jul 31;4(7):e6462.
Price, Lance B., et al. “Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.PLoS One, vol. 4, no. 7, July 2009, p. e6462. Pubmed, doi:10.1371/journal.pone.0006462.
Price LB, Liu CM, Melendez JH, Frankel YM, Engelthaler D, Aziz M, Bowers J, Rattray R, Ravel J, Kingsley C, Keim PS, Lazarus GS, Zenilman JM. Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One. 2009 Jul 31;4(7):e6462.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

July 31, 2009

Volume

4

Issue

7

Start / End Page

e6462

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • RNA, Ribosomal, 16S
  • Middle Aged
  • Microbial Sensitivity Tests
  • Male
  • Humans
  • General Science & Technology
  • Female
  • Diabetes Mellitus
  • Cohort Studies