Skip to main content

Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip.

Publication ,  Journal Article
Musah, S; Dimitrakakis, N; Camacho, DM; Church, GM; Ingber, DE
Published in: Nature protocols
July 2018

Protocols have been established to direct the differentiation of human induced pluripotent stem (iPS) cells into nephron progenitor cells and organoids containing many types of kidney cells, but it has been difficult to direct the differentiation of iPS cells to form specific types of mature human kidney cells with high yield. Here, we describe a detailed protocol for the directed differentiation of human iPS cells into mature, post-mitotic kidney glomerular podocytes with high (>90%) efficiency within 26 d and under chemically defined conditions, without genetic manipulations or subpopulation selection. We also describe how these iPS cell-derived podocytes may be induced to form within a microfluidic organ-on-a-chip (Organ Chip) culture device to build a human kidney Glomerulus Chip that mimics the structure and function of the kidney glomerular capillary wall in vitro within 35 d (starting with undifferentiated iPS cells). The podocyte differentiation protocol requires skills for culturing iPS cells, and the development of a Glomerulus Chip requires some experience with building and operating microfluidic cell culture systems. This method could be useful for applications in nephrotoxicity screening, therapeutic development, and regenerative medicine, as well as mechanistic study of kidney development and disease.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nature protocols

DOI

EISSN

1750-2799

ISSN

1754-2189

Publication Date

July 2018

Volume

13

Issue

7

Start / End Page

1662 / 1685

Related Subject Headings

  • Podocytes
  • Microfluidics
  • Lab-On-A-Chip Devices
  • Kidney Glomerulus
  • Induced Pluripotent Stem Cells
  • Humans
  • Cytological Techniques
  • Cell Differentiation
  • Bioinformatics
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Musah, S., Dimitrakakis, N., Camacho, D. M., Church, G. M., & Ingber, D. E. (2018). Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Nature Protocols, 13(7), 1662–1685. https://doi.org/10.1038/s41596-018-0007-8
Musah, Samira, Nikolaos Dimitrakakis, Diogo M. Camacho, George M. Church, and Donald E. Ingber. “Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip.Nature Protocols 13, no. 7 (July 2018): 1662–85. https://doi.org/10.1038/s41596-018-0007-8.
Musah S, Dimitrakakis N, Camacho DM, Church GM, Ingber DE. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Nature protocols. 2018 Jul;13(7):1662–85.
Musah, Samira, et al. “Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip.Nature Protocols, vol. 13, no. 7, July 2018, pp. 1662–85. Epmc, doi:10.1038/s41596-018-0007-8.
Musah S, Dimitrakakis N, Camacho DM, Church GM, Ingber DE. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Nature protocols. 2018 Jul;13(7):1662–1685.

Published In

Nature protocols

DOI

EISSN

1750-2799

ISSN

1754-2189

Publication Date

July 2018

Volume

13

Issue

7

Start / End Page

1662 / 1685

Related Subject Headings

  • Podocytes
  • Microfluidics
  • Lab-On-A-Chip Devices
  • Kidney Glomerulus
  • Induced Pluripotent Stem Cells
  • Humans
  • Cytological Techniques
  • Cell Differentiation
  • Bioinformatics
  • 11 Medical and Health Sciences