Skip to main content

Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain.

Publication ,  Journal Article
Parisien, M; Samoshkin, A; Tansley, SN; Piltonen, MH; Martin, LJ; El-Hachem, N; Dagostino, C; Allegri, M; Mogil, JS; Khoutorsky, A; Diatchenko, L
Published in: Pain
April 2019

Chronic pain is a debilitating and poorly treated condition whose underlying mechanisms are poorly understood. Nerve injury and inflammation cause alterations in gene expression in tissues associated with pain processing, supporting molecular and cellular mechanisms that maintain painful states. However, it is not known whether transcriptome changes can be used to reconstruct a molecular pathophysiology of pain. In the current study, we identify molecular pathways contributing to chronic pain states through the analysis of global changes in the transcriptome of dorsal root ganglia, spinal cord, brain, and blood in mouse assays of nerve injury- and inflammation-induced pain. Comparative analyses of differentially expressed genes identified substantial similarities between 2 animal pain assays and with human low-back pain. Furthermore, the extracellular matrix (ECM) organization has been found the most commonly regulated pathway across all tested tissues in the 2 animal assays. Examination of human genome-wide association study data sets revealed an overrepresentation of differentially expressed genes within the ECM organization pathway in single nucleotide polymorphisms most strongly associated with human back pain. In summary, our comprehensive transcriptomics analysis in mouse and human identified ECM organization as a central molecular pathway in the development of chronic pain.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Pain

DOI

EISSN

1872-6623

Publication Date

April 2019

Volume

160

Issue

4

Start / End Page

932 / 944

Location

United States

Related Subject Headings

  • Transcriptome
  • RNA, Messenger
  • Polymorphism, Single Nucleotide
  • Pain Measurement
  • Neuralgia
  • Mice, Inbred BALB C
  • Mice
  • Inflammation
  • Humans
  • Genetic Testing
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Parisien, M., Samoshkin, A., Tansley, S. N., Piltonen, M. H., Martin, L. J., El-Hachem, N., … Diatchenko, L. (2019). Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain. Pain, 160(4), 932–944. https://doi.org/10.1097/j.pain.0000000000001471
Parisien, Marc, Alexander Samoshkin, Shannon N. Tansley, Marjo H. Piltonen, Loren J. Martin, Nehme El-Hachem, Concetta Dagostino, et al. “Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain.Pain 160, no. 4 (April 2019): 932–44. https://doi.org/10.1097/j.pain.0000000000001471.
Parisien M, Samoshkin A, Tansley SN, Piltonen MH, Martin LJ, El-Hachem N, et al. Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain. Pain. 2019 Apr;160(4):932–44.
Parisien, Marc, et al. “Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain.Pain, vol. 160, no. 4, Apr. 2019, pp. 932–44. Pubmed, doi:10.1097/j.pain.0000000000001471.
Parisien M, Samoshkin A, Tansley SN, Piltonen MH, Martin LJ, El-Hachem N, Dagostino C, Allegri M, Mogil JS, Khoutorsky A, Diatchenko L. Genetic pathway analysis reveals a major role for extracellular matrix organization in inflammatory and neuropathic pain. Pain. 2019 Apr;160(4):932–944.

Published In

Pain

DOI

EISSN

1872-6623

Publication Date

April 2019

Volume

160

Issue

4

Start / End Page

932 / 944

Location

United States

Related Subject Headings

  • Transcriptome
  • RNA, Messenger
  • Polymorphism, Single Nucleotide
  • Pain Measurement
  • Neuralgia
  • Mice, Inbred BALB C
  • Mice
  • Inflammation
  • Humans
  • Genetic Testing