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Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.

Publication ,  Journal Article
Resendez, SL; Jennings, JH; Ung, RL; Namboodiri, VMK; Zhou, ZC; Otis, JM; Nomura, H; McHenry, JA; Kosyk, O; Stuber, GD
Published in: Nature protocols
March 2016

Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly expanded our understanding of the brain. However, owing to the light-scattering properties of the brain, as well as the size and rigidity of traditional imaging technology, in vivo calcium imaging has been limited to superficial brain structures during head-fixed behavioral tasks. These limitations can now be circumvented by using miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thus permitting optical access to deep brain (or superficial) neural ensembles during naturalistic behaviors. Here we describe steps to conduct such imaging studies using mice. However, we anticipate that the protocol can be easily adapted for use in other small vertebrates. Successful completion of this protocol will permit cellular imaging of neuronal activity and the generation of data sets with sufficient statistical power to correlate neural activity with stimulus presentation, physiological state and other aspects of complex behavioral tasks. This protocol takes 6-11 weeks to complete.

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Published In

Nature protocols

DOI

EISSN

1750-2799

ISSN

1754-2189

Publication Date

March 2016

Volume

11

Issue

3

Start / End Page

566 / 597

Related Subject Headings

  • Prostheses and Implants
  • Optical Imaging
  • Neurons
  • Nerve Net
  • Miniaturization
  • Microscopy
  • Mice
  • Male
  • Equipment Design
  • Endoscopes
 

Citation

APA
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ICMJE
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Resendez, S. L., Jennings, J. H., Ung, R. L., Namboodiri, V. M. K., Zhou, Z. C., Otis, J. M., … Stuber, G. D. (2016). Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. Nature Protocols, 11(3), 566–597. https://doi.org/10.1038/nprot.2016.021
Resendez, Shanna L., Josh H. Jennings, Randall L. Ung, Vijay Mohan K. Namboodiri, Zhe Charles Zhou, James M. Otis, Hiroshi Nomura, Jenna A. McHenry, Oksana Kosyk, and Garret D. Stuber. “Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.Nature Protocols 11, no. 3 (March 2016): 566–97. https://doi.org/10.1038/nprot.2016.021.
Resendez, Shanna L., et al. “Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.Nature Protocols, vol. 11, no. 3, Mar. 2016, pp. 566–97. Epmc, doi:10.1038/nprot.2016.021.
Resendez SL, Jennings JH, Ung RL, Namboodiri VMK, Zhou ZC, Otis JM, Nomura H, McHenry JA, Kosyk O, Stuber GD. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. Nature protocols. 2016 Mar;11(3):566–597.

Published In

Nature protocols

DOI

EISSN

1750-2799

ISSN

1754-2189

Publication Date

March 2016

Volume

11

Issue

3

Start / End Page

566 / 597

Related Subject Headings

  • Prostheses and Implants
  • Optical Imaging
  • Neurons
  • Nerve Net
  • Miniaturization
  • Microscopy
  • Mice
  • Male
  • Equipment Design
  • Endoscopes