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Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors.

Publication ,  Journal Article
Tavares Pereira, M; Gram, A; Nowaczyk, R; Boos, A; Hoffmann, B; Janowski, T; Kowalewski, MP
Published in: Reproductive biology
March 2019

Prostaglandins (PGs) are important regulators of the early corpus luteum (CL) in the dog. Whereas, initially, CL is gonadotropin independent, in the second half of its lifespan, hypophyseal support is required. The transition period is marked by decreased availability of PGs, in particular of PGE2. We previously reported that inhibition of COX2/PTGS2 in vivo suppressed luteal production of PGE2, lowered circulating progesterone and negatively affected luteal development. Therefore, bitches were treated with a COX2-specific blocker, firocoxib, for 5, 10, 20 and 30 days after ovulation, leading to suppression of the steroidogenic machinery. Control groups received a placebo for the same periods. Considering the wide range of possible modulatory roles of PGs shown in different organ systems, this follow-up project aimed to understand further possible PG-mediated effects in early canine CL. Thirty-four (34) factors related predominantly to vascularization and immune response were screened (mRNAs and proteins) on samples from the above described in vivo study. Most of the effects were observed during the transitional period (days 20 and 30). The inhibition of COX2 diminished the expression of angiopoietin family members ANGPT1, -2, Tie1 and -2 receptors. The expression of endothelin (ET)-1 was increased. Concerning the immune system, increased expression of the pro-inflammatory cytokines, IL1β, IL6 and IL12a, and elevated expression levels of CD4, was observed. Cumulatively, besides its involvement in regulating steroidogenesis, our results indicate a broader role of PGs in the canine CL, including modulation of angiogenesis, vascular stabilization and local immunomodulation. Possible cross-species translational effects are strongly implied.

Duke Scholars

Published In

Reproductive biology

DOI

EISSN

2300-732X

ISSN

1642-431X

Publication Date

March 2019

Volume

19

Issue

1

Start / End Page

100 / 111

Related Subject Headings

  • Transcriptome
  • Sulfones
  • Receptors, Estrogen
  • RNA, Messenger
  • Prostaglandins
  • Obstetrics & Reproductive Medicine
  • Immunologic Factors
  • Gene Expression Regulation
  • Female
  • Dogs
 

Citation

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Tavares Pereira, M., Gram, A., Nowaczyk, R., Boos, A., Hoffmann, B., Janowski, T., & Kowalewski, M. P. (2019). Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors. Reproductive Biology, 19(1), 100–111. https://doi.org/10.1016/j.repbio.2019.02.001
Tavares Pereira, Miguel, Aykut Gram, Renata Nowaczyk, Alois Boos, Bernd Hoffmann, Tomasz Janowski, and Mariusz P. Kowalewski. “Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors.Reproductive Biology 19, no. 1 (March 2019): 100–111. https://doi.org/10.1016/j.repbio.2019.02.001.
Tavares Pereira M, Gram A, Nowaczyk R, Boos A, Hoffmann B, Janowski T, et al. Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors. Reproductive biology. 2019 Mar;19(1):100–11.
Tavares Pereira, Miguel, et al. “Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors.Reproductive Biology, vol. 19, no. 1, Mar. 2019, pp. 100–11. Epmc, doi:10.1016/j.repbio.2019.02.001.
Tavares Pereira M, Gram A, Nowaczyk R, Boos A, Hoffmann B, Janowski T, Kowalewski MP. Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors. Reproductive biology. 2019 Mar;19(1):100–111.
Journal cover image

Published In

Reproductive biology

DOI

EISSN

2300-732X

ISSN

1642-431X

Publication Date

March 2019

Volume

19

Issue

1

Start / End Page

100 / 111

Related Subject Headings

  • Transcriptome
  • Sulfones
  • Receptors, Estrogen
  • RNA, Messenger
  • Prostaglandins
  • Obstetrics & Reproductive Medicine
  • Immunologic Factors
  • Gene Expression Regulation
  • Female
  • Dogs