An artery-specific fluorescent dye for studying neurovascular coupling.
Publication
, Journal Article
Shen, Z; Lu, Z; Chhatbar, PY; O'Herron, P; Kara, P
Published in: Nat Methods
January 22, 2012
We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.
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Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
January 22, 2012
Volume
9
Issue
3
Start / End Page
273 / 276
Location
United States
Related Subject Headings
- Voltage-Sensitive Dye Imaging
- Rats
- Neurons
- Microscopy, Fluorescence, Multiphoton
- Mice
- Fluorescent Dyes
- Developmental Biology
- Cerebral Arteries
- Cats
- Brain
Citation
APA
Chicago
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MLA
NLM
Shen, Z., Lu, Z., Chhatbar, P. Y., O’Herron, P., & Kara, P. (2012). An artery-specific fluorescent dye for studying neurovascular coupling. Nat Methods, 9(3), 273–276. https://doi.org/10.1038/nmeth.1857
Shen, Zhiming, Zhongyang Lu, Pratik Y. Chhatbar, Philip O’Herron, and Prakash Kara. “An artery-specific fluorescent dye for studying neurovascular coupling.” Nat Methods 9, no. 3 (January 22, 2012): 273–76. https://doi.org/10.1038/nmeth.1857.
Shen Z, Lu Z, Chhatbar PY, O’Herron P, Kara P. An artery-specific fluorescent dye for studying neurovascular coupling. Nat Methods. 2012 Jan 22;9(3):273–6.
Shen, Zhiming, et al. “An artery-specific fluorescent dye for studying neurovascular coupling.” Nat Methods, vol. 9, no. 3, Jan. 2012, pp. 273–76. Pubmed, doi:10.1038/nmeth.1857.
Shen Z, Lu Z, Chhatbar PY, O’Herron P, Kara P. An artery-specific fluorescent dye for studying neurovascular coupling. Nat Methods. 2012 Jan 22;9(3):273–276.
Published In
Nat Methods
DOI
EISSN
1548-7105
Publication Date
January 22, 2012
Volume
9
Issue
3
Start / End Page
273 / 276
Location
United States
Related Subject Headings
- Voltage-Sensitive Dye Imaging
- Rats
- Neurons
- Microscopy, Fluorescence, Multiphoton
- Mice
- Fluorescent Dyes
- Developmental Biology
- Cerebral Arteries
- Cats
- Brain