An artery-specific fluorescent dye for studying neurovascular coupling.
Publication
, Journal Article
Shen, Z; Lu, Z; Chhatbar, PY; O'Herron, P; Kara, P
Published in: Nature methods
January 2012
We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.
Duke Scholars
Published In
Nature methods
DOI
EISSN
1548-7105
ISSN
1548-7091
Publication Date
January 2012
Volume
9
Issue
3
Start / End Page
273 / 276
Related Subject Headings
- Voltage-Sensitive Dye Imaging
- Rats
- Neurons
- Microscopy, Fluorescence, Multiphoton
- Mice
- Fluorescent Dyes
- Developmental Biology
- Cerebral Arteries
- Cats
- Brain
Citation
APA
Chicago
ICMJE
MLA
NLM
Shen, Z., Lu, Z., Chhatbar, P. Y., O’Herron, P., & Kara, P. (2012). An artery-specific fluorescent dye for studying neurovascular coupling. Nature Methods, 9(3), 273–276. https://doi.org/10.1038/nmeth.1857
Shen, Zhiming, Zhongyang Lu, Pratik Y. Chhatbar, Philip O’Herron, and Prakash Kara. “An artery-specific fluorescent dye for studying neurovascular coupling.” Nature Methods 9, no. 3 (January 2012): 273–76. https://doi.org/10.1038/nmeth.1857.
Shen Z, Lu Z, Chhatbar PY, O’Herron P, Kara P. An artery-specific fluorescent dye for studying neurovascular coupling. Nature methods. 2012 Jan;9(3):273–6.
Shen, Zhiming, et al. “An artery-specific fluorescent dye for studying neurovascular coupling.” Nature Methods, vol. 9, no. 3, Jan. 2012, pp. 273–76. Epmc, doi:10.1038/nmeth.1857.
Shen Z, Lu Z, Chhatbar PY, O’Herron P, Kara P. An artery-specific fluorescent dye for studying neurovascular coupling. Nature methods. 2012 Jan;9(3):273–276.
Published In
Nature methods
DOI
EISSN
1548-7105
ISSN
1548-7091
Publication Date
January 2012
Volume
9
Issue
3
Start / End Page
273 / 276
Related Subject Headings
- Voltage-Sensitive Dye Imaging
- Rats
- Neurons
- Microscopy, Fluorescence, Multiphoton
- Mice
- Fluorescent Dyes
- Developmental Biology
- Cerebral Arteries
- Cats
- Brain