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Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG).

Publication ,  Journal Article
Sharer, JD; De Biase, I; Matern, D; Young, S; Bennett, MJ; Tolun, AA; ACMG Laboratory Quality Assurance Committee,
Published in: Genet Med
December 2018

Amino acid abnormalities are observed in a broad spectrum of inherited metabolic diseases, such as disorders of amino acid metabolism and transport, organic acidemias, and ureagenesis defects. Comprehensive analysis of physiologic amino acids in blood, urine, and cerebrospinal fluid is typically performed in the following clinical settings: evaluation of symptomatic patients in whom a diagnosis is not known; evaluation of previously diagnosed patients to monitor treatment efficacy; evaluation of asymptomatic or presymptomatic (at-risk) relatives of known patients; follow-up testing for an abnormal newborn screen; and assessment of dietary protein adequacy or renal function in general patient populations. Currently, the most common analytical method to quantify amino acids is based on ion exchange chromatography using post-column derivatization with ninhydrin and spectrophotometric detection. Newer methodologies are based on liquid chromatographic separation with detection by mass spectrometry or spectrophotometry. Amino acid analysis by nonseparation methods, such as the flow injection-tandem mass spectrometric (MS/MS) method used for newborn screening, is considered inadequate for the diagnosis of at-risk patients. The purpose of this document is to provide a technical standard for amino acid analysis as applied to the diagnosis and management of inborn errors of metabolism.

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Published In

Genet Med

DOI

EISSN

1530-0366

Publication Date

December 2018

Volume

20

Issue

12

Start / End Page

1499 / 1507

Location

United States

Related Subject Headings

  • United States
  • Tandem Mass Spectrometry
  • Neonatal Screening
  • Metabolism, Inborn Errors
  • Infant, Newborn
  • Humans
  • Genomics
  • Genetics, Medical
  • Genetics & Heredity
  • Chromatography, Liquid
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sharer, J. D., De Biase, I., Matern, D., Young, S., Bennett, M. J., Tolun, A. A., & ACMG Laboratory Quality AssuranceCommittee, . (2018). Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG). Genet Med, 20(12), 1499–1507. https://doi.org/10.1038/s41436-018-0328-6
Sharer, J Daniel, Irene De Biase, Dietrich Matern, Sarah Young, Michael J. Bennett, Adviye A. Tolun, and Adviye A. ACMG Laboratory Quality AssuranceCommittee. “Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG).Genet Med 20, no. 12 (December 2018): 1499–1507. https://doi.org/10.1038/s41436-018-0328-6.
Sharer JD, De Biase I, Matern D, Young S, Bennett MJ, Tolun AA, et al. Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG). Genet Med. 2018 Dec;20(12):1499–507.
Sharer, J. Daniel, et al. “Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG).Genet Med, vol. 20, no. 12, Dec. 2018, pp. 1499–507. Pubmed, doi:10.1038/s41436-018-0328-6.
Sharer JD, De Biase I, Matern D, Young S, Bennett MJ, Tolun AA, ACMG Laboratory Quality AssuranceCommittee. Laboratory analysis of amino acids, 2018 revision: a technical standard of the American College of Medical Genetics and Genomics (ACMG). Genet Med. 2018 Dec;20(12):1499–1507.

Published In

Genet Med

DOI

EISSN

1530-0366

Publication Date

December 2018

Volume

20

Issue

12

Start / End Page

1499 / 1507

Location

United States

Related Subject Headings

  • United States
  • Tandem Mass Spectrometry
  • Neonatal Screening
  • Metabolism, Inborn Errors
  • Infant, Newborn
  • Humans
  • Genomics
  • Genetics, Medical
  • Genetics & Heredity
  • Chromatography, Liquid