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Global analysis of gene expression in renal ischemia-reperfusion in the mouse.

Publication ,  Journal Article
Yoshida, T; Tang, S-S; Hsiao, L-L; Jensen, RV; Ingelfinger, JR; Gullans, SR
Published in: Biochem Biophys Res Commun
March 8, 2002

Ischemia-induced acute renal failure (ARF) is a relatively common disorder with major morbidity and mortality. To study global gene expression during ARF, 6-week-old C57BL/6 male mice underwent 30 min of bilateral renal ischemia followed by reperfusion [I/R] or sham operation. Oligonucleotide microarrays [Affymetrix] with approximately 10,000 genes, 6,643 of which were present in mouse kidney, were used to analyze mRNA expression for up to 4 days following I/R. Fifty-two genes at day 1 and 40 at day 4 were up-regulated more than 4-fold [400%]. Seventy genes at day 1 and 30 genes at day 4 were down-regulated to under 0.25-fold from baseline [25%]. Real-time quantitative RT-PCR confirmed changes in expression for 8 genes of interest. Most of the induced transcripts are involved in cell structure, extracellular matrix, intracellular calcium binding, and cell division/differentiation. Our data identified several novel genes that may be important in renal repair after ischemia.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 8, 2002

Volume

291

Issue

4

Start / End Page

787 / 794

Location

United States

Related Subject Headings

  • Wound Healing
  • Up-Regulation
  • Signal Transduction
  • Reperfusion Injury
  • RNA
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Kinetics
 

Citation

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Yoshida, T., Tang, S.-S., Hsiao, L.-L., Jensen, R. V., Ingelfinger, J. R., & Gullans, S. R. (2002). Global analysis of gene expression in renal ischemia-reperfusion in the mouse. Biochem Biophys Res Commun, 291(4), 787–794. https://doi.org/10.1006/bbrc.2002.6535
Yoshida, Takumi, Shiow-Shih Tang, Li-Li Hsiao, Roderick V. Jensen, Julie R. Ingelfinger, and Steven R. Gullans. “Global analysis of gene expression in renal ischemia-reperfusion in the mouse.Biochem Biophys Res Commun 291, no. 4 (March 8, 2002): 787–94. https://doi.org/10.1006/bbrc.2002.6535.
Yoshida T, Tang S-S, Hsiao L-L, Jensen RV, Ingelfinger JR, Gullans SR. Global analysis of gene expression in renal ischemia-reperfusion in the mouse. Biochem Biophys Res Commun. 2002 Mar 8;291(4):787–94.
Yoshida, Takumi, et al. “Global analysis of gene expression in renal ischemia-reperfusion in the mouse.Biochem Biophys Res Commun, vol. 291, no. 4, Mar. 2002, pp. 787–94. Pubmed, doi:10.1006/bbrc.2002.6535.
Yoshida T, Tang S-S, Hsiao L-L, Jensen RV, Ingelfinger JR, Gullans SR. Global analysis of gene expression in renal ischemia-reperfusion in the mouse. Biochem Biophys Res Commun. 2002 Mar 8;291(4):787–794.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

March 8, 2002

Volume

291

Issue

4

Start / End Page

787 / 794

Location

United States

Related Subject Headings

  • Wound Healing
  • Up-Regulation
  • Signal Transduction
  • Reperfusion Injury
  • RNA
  • Oligonucleotide Array Sequence Analysis
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Kinetics