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Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection.

Publication ,  Journal Article
Aizawa, T; Ishizaka, N; Taguchi, JI; Nagai, R; Mori, I; Tang, SS; Ingelfinger, JR; Ohno, M
Published in: Hypertension
March 2000

In this study, we investigated the regulation and physiological role of heme oxygenase-1 (HO-1) in the kidney of rats with hypertension. Rats were continuously administered either angiotensin II (Ang II) or norepinephrine with an osmotic minipump for up to 7 days. Ang II infusion decreased the glomerular filtration rate (GFR) as determined through creatinine clearance (3.2+/-0.2 versus 1.2+/-0.2 mL/min with Ang II infusion, P<0.01) and increased proteinuria (9. 7+/-1.3 versus 28.1+/-7.2 mg/d with Ang II infusion, P<0.01). In contrast, norepinephrine did not alter these laboratory values. Ang II infusion significantly increased HO-1 expression in mRNA (442+/-98% of control at day 5, P<0.01) and protein levels (314+/-49% of control at day 5, P<0.01). Immunohistochemistry showed that in the kidney of normotensive rats, HO-1 was expressed mainly in the basal side in the renal tubules. After Ang II infusion, HO-1 staining was more extensively dispersed in the tubular epithelial cells. The intraperitoneal administration of zinc protoporphyrin, an HO inhibitor, to Ang II-infused rats further decreased GFR (0.8+/-0. 1 mL/min) and increased proteinuria (52.5+/-13.0 mg/d). In contrast, the administration of hemin, an HO inducer, ameliorated the Ang II-induced decrease in GFR (2.4+/-0.2 mL/min) and increase in proteinuria (9.3+/-4.5 mg/d). These data suggest that HO-1 upregulation in the kidney of Ang II-induced hypertensive rats may exert a renoprotective effect against Ang II-induced renal injury.

Duke Scholars

Published In

Hypertension

DOI

EISSN

1524-4563

Publication Date

March 2000

Volume

35

Issue

3

Start / End Page

800 / 806

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Vasoconstrictor Agents
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protoporphyrins
  • Proteinuria
  • Oxidative Stress
  • Norepinephrine
  • Losartan
 

Citation

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ICMJE
MLA
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Aizawa, T., Ishizaka, N., Taguchi, J. I., Nagai, R., Mori, I., Tang, S. S., … Ohno, M. (2000). Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection. Hypertension, 35(3), 800–806. https://doi.org/10.1161/01.hyp.35.3.800
Aizawa, T., N. Ishizaka, J. I. Taguchi, R. Nagai, I. Mori, S. S. Tang, J. R. Ingelfinger, and M. Ohno. “Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection.Hypertension 35, no. 3 (March 2000): 800–806. https://doi.org/10.1161/01.hyp.35.3.800.
Aizawa T, Ishizaka N, Taguchi JI, Nagai R, Mori I, Tang SS, et al. Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection. Hypertension. 2000 Mar;35(3):800–6.
Aizawa, T., et al. “Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection.Hypertension, vol. 35, no. 3, Mar. 2000, pp. 800–06. Pubmed, doi:10.1161/01.hyp.35.3.800.
Aizawa T, Ishizaka N, Taguchi JI, Nagai R, Mori I, Tang SS, Ingelfinger JR, Ohno M. Heme oxygenase-1 is upregulated in the kidney of angiotensin II-induced hypertensive rats : possible role in renoprotection. Hypertension. 2000 Mar;35(3):800–806.

Published In

Hypertension

DOI

EISSN

1524-4563

Publication Date

March 2000

Volume

35

Issue

3

Start / End Page

800 / 806

Location

United States

Related Subject Headings

  • Vasodilator Agents
  • Vasoconstrictor Agents
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protoporphyrins
  • Proteinuria
  • Oxidative Stress
  • Norepinephrine
  • Losartan