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Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells.

Publication ,  Journal Article
Ottaviano, FG; Tang, S-S; Handy, DE; Loscalzo, J
Published in: Mol Cell Biochem
July 2009

Plasma glutathione peroxidase (GPx-3) is a selenocysteine-containing extracellular antioxidant protein that catalyzes the reduction of hydrogen peroxide and lipid hydroperoxides. Selenoprotein expression involves the alternate recognition of a UGA codon as a selenocysteine codon and requires signals in the 3'-untranslated region (UTR), including a selenocysteine insertion sequence (SECIS), as well as specific translational cofactors. To ascertain regulatory determinants of GPx-3 expression and function, we generated recombinant GPx-3 (rGPX-3) constructs with various 3'-UTR, as well as a Sec73Cys mutant. In transfected Cos7 cells, the Sec73Cys mutant was expressed at higher levels than the wild type rGPx-3, although the wild type rGPx-3 had higher specific activity, similar to plasma purified GPx-3. A 3'-UTR with only the SECIS was insufficient for wild type rGPx-3 protein expression. Selenocompound supplementation and co-transfection with SECIS binding protein 2 increased wild type rGPx-3 expression. These results demonstrate the importance of translational mechanisms in GPx-3 expression.

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Published In

Mol Cell Biochem

DOI

EISSN

1573-4919

Publication Date

July 2009

Volume

327

Issue

1-2

Start / End Page

111 / 126

Location

Netherlands

Related Subject Headings

  • Transfection
  • Selenoproteins
  • Selenium
  • Recombinant Proteins
  • Humans
  • Glutathione Peroxidase
  • Chlorocebus aethiops
  • Cells, Cultured
  • COS Cells
  • Biochemistry & Molecular Biology
 

Citation

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Ottaviano, F. G., Tang, S.-S., Handy, D. E., & Loscalzo, J. (2009). Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells. Mol Cell Biochem, 327(1–2), 111–126. https://doi.org/10.1007/s11010-009-0049-x
Ottaviano, Filomena G., Shiow-Shih Tang, Diane E. Handy, and Joseph Loscalzo. “Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells.Mol Cell Biochem 327, no. 1–2 (July 2009): 111–26. https://doi.org/10.1007/s11010-009-0049-x.
Ottaviano FG, Tang S-S, Handy DE, Loscalzo J. Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells. Mol Cell Biochem. 2009 Jul;327(1–2):111–26.
Ottaviano, Filomena G., et al. “Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells.Mol Cell Biochem, vol. 327, no. 1–2, July 2009, pp. 111–26. Pubmed, doi:10.1007/s11010-009-0049-x.
Ottaviano FG, Tang S-S, Handy DE, Loscalzo J. Regulation of the extracellular antioxidant selenoprotein plasma glutathione peroxidase (GPx-3) in mammalian cells. Mol Cell Biochem. 2009 Jul;327(1–2):111–126.
Journal cover image

Published In

Mol Cell Biochem

DOI

EISSN

1573-4919

Publication Date

July 2009

Volume

327

Issue

1-2

Start / End Page

111 / 126

Location

Netherlands

Related Subject Headings

  • Transfection
  • Selenoproteins
  • Selenium
  • Recombinant Proteins
  • Humans
  • Glutathione Peroxidase
  • Chlorocebus aethiops
  • Cells, Cultured
  • COS Cells
  • Biochemistry & Molecular Biology