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Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution.

Publication ,  Journal Article
Goldstein, BJ; Hare, JM; Lieberman, S; Casiano, R
Published in: Int Forum Allergy Rhinol
July 2013

BACKGROUND: The olfactory epithelium is a self-renewing tissue, able to produce new neurons as needed from stem and progenitor cells in its basal layers. In addition, there exists a mesenchymal-like stem cell (MSC) located within the underlying lamina propria. Little is known about the function of this nasal MSC, or its relationship to the olfactory lineage, but there is considerable interest in using the nasal MSC for cell-based therapies. We sought to further explore the biology of the nasal MSC by establishing neurosphere cultures from adult human nasal biopsies, and to examine the anatomic distribution of nasal MSCs. METHODS: Nasal biopsies from human patients (n = 5) were obtained from superior, middle, and inferior turbinates or septum. Tissue was cultured to obtain nasal MSCs. Cultures were analyzed by immunocytochemistry and flow cytometry, as well as for differentiation capacity. RESULTS: Although olfactory sensory neuroepithelium is restricted to superior regions in the nasal cavity, neurosphere-forming MSC cultures were, surprisingly, obtained from olfactory as well as non-olfactory regions. These MSC cultures exhibit characteristic robust neurosphere formation and express CD90, CD105, STRO-1, and nestin. Nasal MSCs were found to give rise to neuronal-like cells under differentiation conditions. CONCLUSION: The unanticipated broad anatomic distribution of nasal MSCs has implications for cell-based therapy research.

Duke Scholars

Published In

Int Forum Allergy Rhinol

DOI

EISSN

2042-6984

Publication Date

July 2013

Volume

3

Issue

7

Start / End Page

550 / 555

Location

United States

Related Subject Headings

  • Turbinates
  • Neurons
  • Nasal Septum
  • Mesenchymal Stem Cells
  • Humans
  • Cells, Cultured
  • Cell Differentiation
  • Adult
  • 3204 Immunology
  • 3202 Clinical sciences
 

Citation

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Chicago
ICMJE
MLA
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Goldstein, B. J., Hare, J. M., Lieberman, S., & Casiano, R. (2013). Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution. Int Forum Allergy Rhinol, 3(7), 550–555. https://doi.org/10.1002/alr.21153
Goldstein, Bradley J., Joshua M. Hare, Seth Lieberman, and Roy Casiano. “Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution.Int Forum Allergy Rhinol 3, no. 7 (July 2013): 550–55. https://doi.org/10.1002/alr.21153.
Goldstein BJ, Hare JM, Lieberman S, Casiano R. Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution. Int Forum Allergy Rhinol. 2013 Jul;3(7):550–5.
Goldstein, Bradley J., et al. “Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution.Int Forum Allergy Rhinol, vol. 3, no. 7, July 2013, pp. 550–55. Pubmed, doi:10.1002/alr.21153.
Goldstein BJ, Hare JM, Lieberman S, Casiano R. Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution. Int Forum Allergy Rhinol. 2013 Jul;3(7):550–555.
Journal cover image

Published In

Int Forum Allergy Rhinol

DOI

EISSN

2042-6984

Publication Date

July 2013

Volume

3

Issue

7

Start / End Page

550 / 555

Location

United States

Related Subject Headings

  • Turbinates
  • Neurons
  • Nasal Septum
  • Mesenchymal Stem Cells
  • Humans
  • Cells, Cultured
  • Cell Differentiation
  • Adult
  • 3204 Immunology
  • 3202 Clinical sciences