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Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.

Publication ,  Journal Article
Navarro-Mendoza, MI; Pérez-Arques, C; Panchal, S; Nicolás, FE; Mondo, SJ; Ganguly, P; Pangilinan, J; Grigoriev, IV; Heitman, J; Sanyal, K; Garre, V
Published in: Curr Biol
November 18, 2019

Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A is tolerated in only a few organisms, many of which possess holocentric chromosomes. Here, we characterize the consequence of the loss of CENP-A in the fungal kingdom. Mucor circinelloides, an opportunistic human pathogen, lacks CENP-A along with the evolutionarily conserved CENP-C but assembles a monocentric chromosome with a localized kinetochore complex throughout the cell cycle. Mis12 and Dsn1, two conserved kinetochore proteins, were found to co-localize to a short region, one in each of nine large scaffolds, composed of an ∼200-bp AT-rich sequence followed by a centromere-specific conserved motif that echoes the structure of budding yeast point centromeres. Resembling fungal regional centromeres, these core centromere regions are embedded in large genomic expanses devoid of genes yet marked by Grem-LINE1s, a novel retrotransposable element silenced by the Dicer-dependent RNAi pathway. Our results suggest that these hybrid features of point and regional centromeres arose from the absence of CENP-A, thus defining novel mosaic centromeres in this early-diverging fungus.

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Published In

Curr Biol

DOI

EISSN

1879-0445

Publication Date

November 18, 2019

Volume

29

Issue

22

Start / End Page

3791 / 3802.e6

Location

England

Related Subject Headings

  • Mucor
  • Kinetochores
  • Histones
  • Developmental Biology
  • Chromosome Segregation
  • Chromosomal Proteins, Non-Histone
  • Chromatin
  • Centromere Protein A
  • Centromere
  • 52 Psychology
 

Citation

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Navarro-Mendoza, M. I., Pérez-Arques, C., Panchal, S., Nicolás, F. E., Mondo, S. J., Ganguly, P., … Garre, V. (2019). Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres. Curr Biol, 29(22), 3791-3802.e6. https://doi.org/10.1016/j.cub.2019.09.024
Navarro-Mendoza, María Isabel, Carlos Pérez-Arques, Shweta Panchal, Francisco E. Nicolás, Stephen J. Mondo, Promit Ganguly, Jasmyn Pangilinan, et al. “Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.Curr Biol 29, no. 22 (November 18, 2019): 3791-3802.e6. https://doi.org/10.1016/j.cub.2019.09.024.
Navarro-Mendoza MI, Pérez-Arques C, Panchal S, Nicolás FE, Mondo SJ, Ganguly P, et al. Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres. Curr Biol. 2019 Nov 18;29(22):3791-3802.e6.
Navarro-Mendoza, María Isabel, et al. “Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.Curr Biol, vol. 29, no. 22, Nov. 2019, pp. 3791-3802.e6. Pubmed, doi:10.1016/j.cub.2019.09.024.
Navarro-Mendoza MI, Pérez-Arques C, Panchal S, Nicolás FE, Mondo SJ, Ganguly P, Pangilinan J, Grigoriev IV, Heitman J, Sanyal K, Garre V. Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres. Curr Biol. 2019 Nov 18;29(22):3791-3802.e6.
Journal cover image

Published In

Curr Biol

DOI

EISSN

1879-0445

Publication Date

November 18, 2019

Volume

29

Issue

22

Start / End Page

3791 / 3802.e6

Location

England

Related Subject Headings

  • Mucor
  • Kinetochores
  • Histones
  • Developmental Biology
  • Chromosome Segregation
  • Chromosomal Proteins, Non-Histone
  • Chromatin
  • Centromere Protein A
  • Centromere
  • 52 Psychology