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BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation.

Publication ,  Journal Article
Wang, L; Park, P; Zhang, H; La Marca, F; Claeson, A; Than, K; Rahman, S; Lin, C-Y
Published in: Int J Cancer
October 15, 2012

Bone morphogenetic protein-2 (BMP-2), a member of the transforming growth factor superfamily, has been shown to have inhibitory effect on many tumor types. However, the effect of BMP-2 on human renal cell carcinoma (RCC) is still unknown. We previously showed that BMP-2 inhibits tumorigenicity of cancer stem cells in human osteosarcoma OS99-1 cells. Our study investigates the effect of BMP-2 on human RCC using ACHN and Caki-2 cell lines. Three types of BMP receptors were found to be expressed in ACHN and Caki-2 cells. In vitro, BMP-2 was found to inhibit the growth of ACHN and Caki-2 cells. The antiproliferative effect seems to be due to cell cycle arrest in the G1 phase, which was revealed by flow cytometry analysis. Using reverse transcriptase polymerase chain reaction analysis, we demonstrated BMP-2 upregulated osteogenic markers Runx-2 and Collagen Type I gene expression in ACHN and Caki-2 cells. Treatment of ACHN and Caki-2 cells with BMP-2 induced a rapid phosphorylation of Smad1/5/8. In vivo, all animals receiving low number of ACHN (1 × 10(4)) and Caki-2 (5 × 10(4)) cells treated with 30 μg of BMP-2 per animal showed limited tumor growth with significant bone formation, whereas untreated cells developed large tumor masses without bone formation in immunodeficient non-obese diabetic (NOD)/severe combined immunodeficient (SCID) mice. These results suggest that BMP-2 inhibits growth of RCC as well as causes induction of osseous bone formation. Further research is needed to determine the relationship between inhibition of cell proliferation and bone induction.

Duke Scholars

Published In

Int J Cancer

DOI

EISSN

1097-0215

Publication Date

October 15, 2012

Volume

131

Issue

8

Start / End Page

1941 / 1950

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Real-Time Polymerase Chain Reaction
  • RNA, Messenger
  • Phosphorylation
  • Osteogenesis
  • Oncology & Carcinogenesis
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Kidney Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, L., Park, P., Zhang, H., La Marca, F., Claeson, A., Than, K., … Lin, C.-Y. (2012). BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation. Int J Cancer, 131(8), 1941–1950. https://doi.org/10.1002/ijc.27444
Wang, Lin, Paul Park, Huina Zhang, Frank La Marca, Amy Claeson, Khoi Than, Shayan Rahman, and Chia-Ying Lin. “BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation.Int J Cancer 131, no. 8 (October 15, 2012): 1941–50. https://doi.org/10.1002/ijc.27444.
Wang L, Park P, Zhang H, La Marca F, Claeson A, Than K, et al. BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation. Int J Cancer. 2012 Oct 15;131(8):1941–50.
Wang, Lin, et al. “BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation.Int J Cancer, vol. 131, no. 8, Oct. 2012, pp. 1941–50. Pubmed, doi:10.1002/ijc.27444.
Wang L, Park P, Zhang H, La Marca F, Claeson A, Than K, Rahman S, Lin C-Y. BMP-2 inhibits tumor growth of human renal cell carcinoma and induces bone formation. Int J Cancer. 2012 Oct 15;131(8):1941–1950.

Published In

Int J Cancer

DOI

EISSN

1097-0215

Publication Date

October 15, 2012

Volume

131

Issue

8

Start / End Page

1941 / 1950

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Real-Time Polymerase Chain Reaction
  • RNA, Messenger
  • Phosphorylation
  • Osteogenesis
  • Oncology & Carcinogenesis
  • Mice, SCID
  • Mice, Inbred NOD
  • Mice
  • Kidney Neoplasms